摘要
目的:建立15种常见精神类药物在人体使用过程中的监测条件,即蛋白沉淀-高效液相色谱法联合分析法的可行性。方法:0.5 mL血浆样品加入1.5 mL乙腈,旋涡混合后,离心,上清液过滤,滤液直接采用HPLC测定。色谱条件:C18柱,采用梯度洗脱方式,流动相组成为:A.乙腈;B.0.1%磷酸水溶液。梯度洗脱程序:0.0～30.0 min(5%A～45%A,95%B～55%B),30.0～35.0 min(45%A～80%A,55%B～20%B),流速1.0 mL.min-1,检测波长210 nm,进样量20μL,柱温30℃。结果:15种药物平均回收率均大于80%,RSD 0.44%～8.07%。结论:该法乙腈沉淀蛋白的条件简单,具有快速、回收率高且稳定,能够作为血浆中药物检测分析的通用前处理方法;梯度洗脱可以作为血浆样品的首选常规色谱分析方法。
OBJECTIVE To establish a protein precipitation-HPLC method for determination of 15 common-used drugs in plasma. METHODS 0. 5 mL plasma was added with 1.5 mL acetonitrile, vortex mixed and centrifuged, the supernatant was filtrated and then injected into the HPLC system. The HPLC conditions are detailed as following： with C18 column, mobile phase of acetonitrile-0. 1% phosphate water solution by gradient elution, 0. 0 - 30. 0 min（5 % A- 45 % A, 95%B- 55 %B）, 30. 0 ＋ 35.0 min（45%A- 80%A,55%B- 20%B）, the flow rate was 1.0 mL·min^-1. The detection wavelength was set at 210 nm, and the injection volume was 20μL, column temperature was 30℃. RESULTS The average absolute recoveries of 15 drugs tested exceeded 80% with RSD ranging from 0. 44% to 8. 07%. CONCLUSION The results showed that protein precipitation by acetonitrile is suitable for plasma drug analysis.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第15期1254-1257,共4页
Chinese Journal of Hospital Pharmacy
