摘要
用电镜细胞化学和分光光度技术对栉孔扇贝血淋巴中的酚氧化酶（ＰＯ）和髓过氧物酶（ＭＰＯ）进行了研究。结果表明：细胞质中直径分别为３００～４３０ｎｍ和１３０～２００ｎｍ的大、小２种颗粒呈ＰＯ强阳性；次级溶酶体和小颗粒呈ＭＰＯ强阳性。生化测定，血清中存在ＰＯ和ＭＰＯ活性，血细胞仅有ＭＰＯ活性。以ＴＭＢ为底物测得血清中不依赖卤素的ＭＰＯ活力为１０７．２Ｕ，以二乙醇胺为底物测得血清中依赖卤素的ＭＰＯ活力为５３．６Ｕ。血清中ＰＯ和ＭＰＯ的最适ｐＨ分别为６．０和６．５，最适温度分别为４５℃和３５℃。
The phenoloxidase (PO) and myeloperoxidase(MPO)in the haemocytes and haemolymph of the Chlamys frrreri were studied with the method of cytochemical localisation using electron-microscopy and specreophotometer.The results show that 2 kinds of granules,300430 nm and 130200 nm in diameter,in cytoplasm are strongly positive to PO. The secondary lysosomes and the small granules are strongly positive to MPO. The results of biochemical test show that there are PO and MPO in haemolymph and there is only MPO in haemocytes. PO is present in an inactive form (proPO) in haemocyres. The enzyme activities of MPO in haemocytes is 3.5 fold as much as those in the haemolymph. Halide-independent MPO in haemolymph is 107.2 U using TMB as the substrate, and halide-dependent MPO in haemolymph is 53.6 U using diethanolamine as the substrate.The optimum pH of PO and MPO in haemolymph are 6.0 and 6.5 and the optimum temperatures of PO and MPO in haemolymph are 45 and 35.
出处
《中国水产科学》
CAS
CSCD
1999年第2期9-13,共5页
Journal of Fishery Sciences of China
基金
国家攀登计划
关键词
栉孔扇贝
酚氧化酶
髓过氧物酶
酶活性
血淋巴
Chlamys farreri, phenoloxidase,myeloperoxidase, enzymatic activity, haemolymph
