摘要
建立了卷烟烟丝中三个主要多酚化合物（绿原酸、莨菪亭、芸香苷）的超高效液相色谱（UPLC）的快速检测方法。采用微波辅助萃取法（microwave-assisted extraction MAE）分别进行单因素试验和正交试验,确定卷烟烟丝中三个主要多酚类化合物的最佳提取条件：60%（V/V）的甲醇水溶液为提取剂、萃取液温度为94℃,微波辐照时间为1 min、料液比为1：20（g/mL）。微波辅助提取烟丝中多酚回收率在96.5%~105.6%之间,相对标准偏差小于5%,结果令人满意。
A fast microwave-assisted extraction-ultrahigh performance liquid chromatography（MAE-UPLC） method was developed for the simultaneous determination of chlorogenic acid,scopoletin and rutin in cut tobacco.The extraction conditions of polyphenols were determined by single-factor and the orthogonal experiment.The optimal conditions were determined as follows： methanol concentration 60%（V/V）,extraction solvent temperature 94 ℃,microwave treatment time 1min and solid to liquid ratio 1：20（g/mL）,under which the recovery rate of polyphenols in cut tobacco were 96.5%-105.6% and the relative standard deviation was lower than 5%.
出处
《现代食品科技》
EI
CAS
2011年第8期1036-1039,共4页
Modern Food Science and Technology
关键词
烟丝
微波辅助萃取
绿原酸
莨菪亭
芸香苷
cut tobacco
scopoletin
chlorogenic acid
rutin
microwave-assisted extraction
