摘要
目的 探讨HBV -DNA定量在肝病发展及转归过程中的意义 ,以及乙型肝炎病毒标志物 (HBVM)与HBVDNA的相互关系。方法 血清的HBVM测定采用ELISA法 ,HBV -DNA定量利用外标法计算机辅助视频成像处理系统测定。结果 血清HBV -DNA含量随着HBsAg、HBeAg的转阴而下降。“小三阳”组HBVDNA( 1 .66× 1 0 9)显著低于“大三阳”组 ( 3.2 2× 1 0 9) ,P <0 .0 5。慢性肝炎 ,肝硬化 ,肝癌三者的血清HBV -DNA定量逐渐降低。且肝硬化组HBVDNA定量 ( 1 .1 5× 1 0 9)与慢性肝炎组 ( 1 .5 1× 1 0 9)差异非常显著 ,P <0 .0 0 1。结论 乙型肝炎病毒DNA是判断乙肝有否传染性的标准 ,HBV -DNA的定量测定可以准确地判断乙型肝炎患者的演变过程。e抗原转变为e抗体后病毒复制并未停止 ,而只是HBV -DNA复制的下降。肝病患者随着肝脏损害的不断加重 ,其HB -DNA的复制会降低。
Objective: To investigate the significance of HBV-DNA quantitative determination in chronic hepatitis and the relationship between HBV-marker and HBVDNA. Methods: HBVM was determined by ELISA and HBVDNA by PCR (Polymerase Chain Reaction ).Results: The amount of HBVDNA in HBeAg positive group (3.22×10 9) was higher than that in HBeAg negative group(1.66×10 9),P<0.05. The amount of HBVM in chronic hepatitis (1.51×10 9)was higher than that in live cirrhosis (1.15×10 9) ,P<0.001 . Conclusion: HBVDNA quantitative determination could well be used to judge the evolutionary process in hepatitis .The virus did not stop proliferation but only decreased proliferation while HBeAg turned to HBeAb .HBVDNA would be reduced with the developing of liver damage.
出处
《泰山医学院学报》
CAS
1999年第4期313-315,共3页
Journal of Taishan Medical College
