摘要
目的探讨微弧氧化(microarc oxidation,MAO)结合应用于纯钛种植体表面处理的可行性。方法根据对纯钛钛片处理的不同将实验分为对照组(A组,不作处理)、MAO组(B组,纯钛片上进行MAO处理)及MAO加Ⅰ型胶原组(C组,纯钛片上MAO处理后吸附Ⅰ型胶原)。将成骨细胞培养于各组钛片上,通过扫描电镜、MTT法检测不同时间点各组钛片表面的细胞生长及增殖情况,并检测碱性磷酸酶(alkaline phosphatase,ALP)活性。结果扫描电镜显示成骨细胞在C组钛片上细胞黏附情况优于A、B组;MTT法及ALP活性检测示培养3、6d,成骨细胞在C组钛片上的增殖及ALP活性与A、B组比较差异均有统计学意义(P<0.05)。结论 MAO结合Ⅰ型胶原处理的钛片可更有效提高成骨细胞表面附着、增殖,且具有较高的ALP活性。
Objective To study the feasibility of applying microarc oxidation（MAO） with collagen Ⅰ in surface modification of pure titanium.Methods According to different processing methods,the pure titanium was divided into three groups：the control group（without surface modification,group A）,MAO group（with microarc oxidation applied in pure titanium surface modification,group B）,MAO＋Ⅰ group（with microarc oxidation and collagen Ⅰ applied in pure titanium treatment,group C）.Osteoblasts were cultured on the surface of titanium in each group,and the cell proliferation in each group was detected at different time points by scanning electron microscopy and MTT method.Moreover,the activity of alkaline phosphatase（ALP） was also detected.Results Scanning electron microscopy showed that adhesion of osteoblasts for group C was better than group A and group B.MTT method and ALP activity detection indicated that there was a significant difference between group C and group A,B in cell proliferation and ALP activity on the third and sixth day of cultivation（P0.05）.Conclusion MAO with collagen Ⅰ applied in surface modification of pure titanium may increase osteoblast attachment,and promote its proliferation and ALP activity.
出处
《华西医学》
CAS
2011年第7期1038-1042,共5页
West China Medical Journal
基金
贵州省优秀科技教育人才专项资金(201083)~~
关键词
纯钛
成骨细胞
微弧氧化
Ⅰ型胶原
Pure Titanium
Osteoblast
Microarc oxidation
Collagen Ⅰ
