摘要
目的探讨CXCR4、Angs在NPM1突变参与调控的白血病细胞浸润转移中的作用，以期进一步明确NPM1突变在白血病浸润转移中的调控机制。方法通过基因转染构建稳定表达NPM1突变蛋白的K562白血病细胞株（K562-mA）。qRT—PCR检测各组细胞CXCR4、Ang-1／2的mRNA表达水平；Western免疫印迹和流式细胞仪分别检测细胞CXCR4总蛋白和膜蛋白的表达。结果建立了稳定表达NPM突变基因的K562-mA细胞株。与未处理组和空载体转染组相比，K562-mA细胞CXCR4的mRNA和蛋白表达水平显著增高；Ang-1mRNA表达水平明显降低、Ang-2mRNA表达水平明显增高。结论CXCR4、Ang-1／2可能在MPM1突变调控白血病细胞的浸润转移中发挥重要作用。
Objective To investigate the role of CXCR4 and Angs in the invasion phenotype in vitro of leukemic cells with NPM1 mutations, and further study the effect of NPM1 mutations on leukemia infiltration. Methods The pEGFPC1-NPMI-mA plasmid vector was transfected into K562 cells. The cells stably expressing NPMI-mA protein were established, named as K562-mA. The ex- pressions of CXCR4 and Ang-1/2 mRNA were assayed by quantitative Real-Time PCR （qRT-PCR）. The expression of CXCR4 protein was assayed by Western blot and flow cytometry. Results Com- pared with control groups, CXCR4 mRNA and protein expression levels were significantly increased in K562-mA group. The expression of Ang-1 mRNA was markedly decreased, whereas Ang-2 mRNA expression was increased in K562-mA group. Conclusion CXCR4 and Ang-1/2 may play important roles in the invasion phenotype of leukemic cells with NPM1 mutations in vitro.
出处
《医学分子生物学杂志》
CAS
CSCD
2011年第4期294-298,共5页
Journal of Medical Molecular Biology
基金
国家自然科学基金面上项目（No.30872418）,重庆市科委自然科学基金（No.CSTC,2010BB5363）
