摘要
目的研究重组脂联素(AD)腺相关病毒载体对大鼠滑膜成纤维细胞(SFLs)肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和B细胞激活因子(BAFF)mRNA表达的影响。方法采用胶原酶消化法制备大鼠SFLs。携带AD基因的腺相关病毒按不同转染复数(MOI)转染大鼠SFLs,于第7天采用RT-PCR方法检测细胞因子AD、TNF-α、IL-6、BAFF mRNA表达。结果成功培养出大鼠SFLs,转染后的SFLs AD mRNA表达随MOI升高而增加;在MOI=5×105v.g./cell时,TNF-α、IL-6、BAFF mRNA表达最高。结论大鼠SFLs中高表达AD能够促进TNF-α、IL-6和BAFFmRNA表达,提示AD参与炎性反应和B细胞活化,在类风湿关节炎的慢性炎症过程中发挥重要作用。
Objective To investigate the effect of recombinant adeno-associated virus vector of adiponectin（AD） on the mRNA expressions of tumor necrosis factor-α（TNF-α）,interlukin-6（IL-6） and B cell-activating factor belonging to the TNF family（BAFF） in rat synovial fibroblasts（SFLs）.Methods The rat SFLs were prepared by collagenase digestion,and then the adenovirus-mediated AD was transfected into rats SFLs according to different multiplicity of infection（MOI）.Seven days after transfection,the mRNA expressions of AD,TNF-α,IL-6 and BAFF were detected by RT-PCR.ResultsThe rat SFLs were successfully cultured.After transfection,the mRNA expression of AD was significantly increased along with the increasment of MOI.Meanwhile,when MOI was at 5×10^5 v.g./cell,the mRNA expressions of TNF-α,IL-6 and BAFF reached the largest level.ConclusionHigh expression of AD in rat SLFs can promote the mRNA expressions of TNF-α,IL-6 and BAFF,suggesting that AD participates in the inflammatory response and the activition of B cell,and plays an important role in the chronic inflammatory process of rheumatoid arthritis.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第15期1762-1765,共4页
Jiangsu Medical Journal
基金
江苏省自然科学基金(BK2007255)
中华医学会研究基金(08010090087)
江苏省卫生厅开放课题(XK12200905)
关键词
类风湿关节炎
脂联素
Rheumatoid arthritis
Adiponectin
