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IL-13促进肝星状细胞增殖和胶原蛋白表达 被引量：5

IL-13 Promotes Hepatic Stellate Cell Proliferation and Collagen Synthesizing
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摘要 肝星状细胞(hepatic stellate cell,HSC)是肝纤维化发展过程中过量细胞外基质的主要来源。该研究首先利用MTT法检测IL-13实验剂量和时间条件下肝星状细胞增殖情况;然后运用RT-PCR技术检测IL-13对人肝星状细胞LX-2细胞系IL-13Rα1、IL-4Rα、TGF-β和Ⅰ型胶原蛋白转录水平的影响;最后通过羟脯氨酸法定量分析各组细胞培养上清液中的胶原蛋白含量。结果发现:IL-13能促进肝星状细胞的增殖;在不改变IL-13Rα1和IL-4Rα转录水平的同时,对TGF-β和Ⅰ型胶原蛋白mRNA的表达以及细胞总胶原蛋白含量的上调作用均呈现出较为明显的剂量和时间依赖性。 Hepatic stellate cell is the primary source of extra ECM deposition in liver wound healing responses. Here we detected proliferation of LX-2 after exposure to IL-13 in different conditions （0-100 ng/mL×24 h or 0-48 h ×10 ng/mL）. Then we analyzed mRNA expression of IL-13Ra 1, IL-4Rα, TGF-β and collagen type I by RT-PCR. Collagen contents in cell supernatant were also determined by hydroxyproline-assaying. In this study we report that IL-13 could promotes hepatic stellate cell proliferation with no vitiation of IL-13Rα 1 and IL-4Rα gene expression. However, in experimental conditions, IL-13 were shown to unregulated TGF-13 and COLA1 expression as well as collagen synthesizing which in a dose- and time-dependent manner.
作者 陈厚文 吴超 李香龙 郭梦舟 熊志勇 范杰 朱孟博 石小玉
机构地区 南昌大学转化医学研究院 南昌大学第一临床医学院 南昌大学第二临床医学院 南昌大学基础医学院
出处 《中国细胞生物学学报》 CAS CSCD 2011年第8期873-879,共7页 Chinese Journal of Cell Biology
基金 国家自然科学基金(No.30860118) 国家大学生创新性实验计划(No.091040306 No.101040307)资助项目~~
关键词 IL-13 肝星状细胞 LX-2 胶原蛋白 IL-13 hepatic stellate cell LX-2 collagen
分类号 R575.2 [医药卫生—消化系统]
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参考文献17

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共引文献5

同被引文献61

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引证文献5

二级引证文献38

中国细胞生物学学报
2011年 第8期

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