摘要
目的探讨干细胞表面抗原-1(Sca-1)在成肌细胞增殖分化中的作用。方法采用流式荧光细胞分选技术,从C57BL/6小鼠骨骼肌中分离Sca-1+与Sca-1-细胞进行体外培养,使用CCK-8试剂盒检测2种细胞的增殖曲线,Westernblot测定2种细胞在体外培养过程中Sca-1、MyoD、成肌素(Myogenin)的蛋白表达。结果在体外培养的前3 d,Sca-1+与Sca-1-细胞增殖曲线没有明显差别;3 d后,Sca-1-细胞增殖比Sca-1+细胞明显加速。同时,2种细胞在体外培养过程中Sca-1均没有明显表达,而Sca-1+细胞的成肌调节因子表达比Sca-1-细胞显著增加。结论 Sca-1的表达在细胞生长发育过程中具有时段性,与成肌细胞细胞周期的起止有关。
Objective To probe into the function of stem cell antigen-1（Sca-1） in cell proliferation and differentiation.Methods Sca-1＋ and Sca-1-muscle derived cells were separated from C57BL/6 mice by fluorescence-activated cell sorting and then cultured in vitro.After 5 days cells proliferative curve were drawn according CCK-8 experimental results.Western blot also were done to detect Sca-1,MyoD and Myogenin expression in cultured Sca-1＋ and Sca-1-muscle derived cells.Results The difference of the proliferative curve of Sca-1＋ and Sca-1-muscle derived cells cultured 3 days in vitro was not apparent,but Sca-1-muscle derived cells had a accelerated division rate in the follow days compared to the Sca-1＋ muscle derived cells.Sca-1 expression in both cells was not obvious.MyoD and Myogenin expression were stronger in Sca-1＋ than Sca-1-muscle derived cells.Conclusion Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2011年第4期369-371,374,共4页
West China Journal of Stomatology
基金
北京市自然科学基金资助项目(7072029)
山东省科技攻关基金资助项目(2007GG30002035)
