摘要
目的:研究非霍奇金淋巴瘤(NHL)中双链RNA依赖的蛋白激酶(PKR)及核因子-κB/P65(NF-κB/P65)的表达及临床意义。方法:采用免疫组化方法检测45例NHL患者、26例淋巴结反应性增生(RH)患者及15例正常者(CON)组织中PKR和NF-κB/P65的表达水平,分析其与NHL病理特征间的相关性。结果:NHL组PKR表达率为69.05%(29/42),RH组为11.54(3/26),正常组为6.67%(1/15),PKR在NHL中的表达水平较RH组及CON组增高(P<0.05)。NHL中NF-κB/P65阳性表达率为73.80%(31/42),RH组为3.85%(1/26),而CON中无NF-κB/P65阳性表达,NHL组织中NF-κB/P65表达较RH组及CON组增高(P<0.05)。PKR表达与NHL患者性别、年龄、细胞类型、恶性程度及临床分期无关;而NF-κB/P65表达与恶性程度及临床分期有关。结论:PKR在NHL中具有一定蛋白表达,NF-κB/P65异常表达与NHL的发生、发展有关。
Objective： To investigate the expressions of double-stranded RNA-activated protein kinase（PKR） and nuclear factor-kappa B（NF-κB/P65） in non-Hodgkin lymphoma patients.Methods： The expressions of PKR and NF-κB/P65 were detected by immunohistochemical method in 45 cases of NHL,26 cases of reactive hyperplasia of lymphnode（RH） and 15 cases of normal tissue.The relationship between clinical pathological features and the expressions of PK and NF-κB/P65 were analysed.Results： The positive rate of PKR expression was 71.11%（32/45） in NHL tissues,11.54（3/26） in RH tissues and 6.67%（1/15） in CON tissues.The positive rate of PKR expression was higher in NHL group than that of RH and CON group（P0.05）.The positive rate of NF-κB/P65 expression was 75.56%（34/45） in NHL tissues and 3.85%（1/26） in RH tissues,but no expression in normal tissues.The positive rate of NF-κB/P65 expression was higher in NHL group than that of RH and normal groups（P=0.000）.There was no correlation between expression of PKR and the gender,age,pathological types,aggressieness and clinical stage.The expression of NF-κB/P65 was associated with clinacal stage and aggressieness.Conclusion：NHL patients express a certain abundance of PKR protein,the abnormal expression of NF-κB is related to the onset and progression in NHL patients.
出处
《现代肿瘤医学》
CAS
2011年第8期1666-1668,共3页
Journal of Modern Oncology
关键词
非霍奇金淋巴瘤
蛋白激酶
核因子-κB/P65
凋亡
non-Hodgkin lymphoma
double-stranded RNA-activated protein kinase
nuclear factor-kappa B/P65
apoptosis
