摘要
【目的】探索鹅源草酸青霉产果胶酶的作用机理,确定其添加效果与使用方法;同时,为果胶酶的推广应用提供理论依据和技术支撑。【方法】选用1日龄同批孵化、健康肉鸡240只,随机分成4个处理组。1组为对照组,饲喂正常水平的基础日粮;2组在基础日粮的基础上添加0.249%的果胶酶;3组添加0.168%纤维素酶;4组添加0.15%由果胶酶和纤维素酶按1﹕1配合而成的复合酶。【结果】果胶酶组肉鸡。显著提高对磷(P)、粗纤维(CF)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、蛋氨酸(Met)、赖氨酸(Lys)、胱氨酸(Cys)、苏氨酸(Thr)和异亮氨酸(Ile)的利用率(P<0.05),极显著提高粗蛋白(CP)的利用率(P<0.01);显著提高果胶酶组28日龄时十二指肠、胰腺的淀粉酶、脂肪酶、胰蛋白酶活性以及空肠的脂肪酶活性(P<0.05),极显著提高胃蛋白酶活性(P<0.01),显著提高49日龄时胰腺的淀粉酶及脂肪酶和胰蛋白酶活性、十二指肠的淀粉酶和胰蛋白酶活性和空肠的脂肪酶和胃蛋白酶活性(P<0.05);显著提高空肠的绒毛高度、降低肠壁厚度(P<0.05);果胶酶组显著降低49日龄的尿酸、尿素氮水平(P<0.05);显著提高的血糖水平(P<0.05);显著降低盲肠大肠杆菌、沙门氏菌数量(P<0.05);显著提高乳酸杆菌和双歧杆菌数量(P<0.05);显著提高49日龄时的新城疫抗体效价(P<0.05)。【结论】日粮中添加鹅源草酸青霉产果胶酶能提高肉鸡的生产性能和养分利用率,改善肠道形态结构、消化酶活性和微生态环境。
[Objective] The objective of the experiment is to study the mechanism of pectinase produced by penicillium oxalicum Currie and Thom from goose, to determine the adding effects and the usage of pectinase in broiler diet, and to provide a theoretical basis and a technical support for the application ofpectinase. [Method] Two hundred and forty healthy 1-day-old broiler chickens under same condition were randomly divided into 4 groups. Group 1 was the control group fed normal level of basal diet The other three groups＇ diets were added with, respectively, pectinase at 0.249%, cellulose at 0.168%, compound enzyme at 0.15% prepared with pectinase and cellulose in proportion of 1 ： 1. [Result] The growth of broilers in pectinase group was significant, pectinase improved the digestibility of P, CF, NDF, ADF, Met, Lys, Cys, Thr, and lie significantly （P〈0.05）, and the digestibility of CP was improved by 17.31% remarkably （P〈0.01）. On the 28th day, compared with the control group, pectinase group＇s amylase, lipase, trypsin activities of duodenum and pancreas （P〈0.05）, lipase activities of jejunum were significantly increased, pepsin activity was also higher than that of control group （P〈 0.01）. On the 49th day pectinase group＇s amylase, lipase, trypsin activities of pancreas, amylase, trypsin activities of duodenum, lipase activity ofj ejunum and pepsin activity were significantly higher than that of the control group （P〈0.05）, jejunum＇s villous height was increased significantly （P〈0.05） and the thickness of intestinal wall was decreased significantly （P〈0.05）. Pectinase group＇s uric acid level and urea nitrogen level significantly decreased during 0-28 days and 29-49 days, blood glucose level significantly increased on 49th day （P〈0.05）, the numbers ofEscherichia coli and Salmonella significantly decreased （P〈0.05）, the numbers of Lactobacillus and Bacillus bifidus significantly increased （P〈0.05）. Serum antibody titres to NDV of 49-day-old significantly increased （P〈0.05）. [Conclusion] The pectinase produced by penicillium oxalicum Currie and Thom from goose could increase the production of broiler performance and nutrient utilization, and improve the gut morphology, digestive enzyme activities and micro-ecological environment.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第15期3223-3234,共12页
Scientia Agricultura Sinica
基金
国家水禽产业技术体系专项基金(CYCYTX-45-07)
关键词
鹅源草酸青霉产果胶酶
肉鸡
生长发育
消化生理
pectinase produced by penicillium oxalicum Currie and Thom
broiler
growth
digestive physiology
