摘要
目的:研究延衰合剂(Yanshuai Mixture,YSM)对D-半乳糖所致亚急性衰老小鼠脾脏组织结构及血清白介素-2(IL-2)水平的影响。方法:用D-半乳糖(D-galactose,D-gal 120 mg.kg-1.d-1)连续sc 8周建立衰老模型小鼠,造模同时用YSMig,观察衰老小鼠脾脏指数、脾脏病理改变、酶联免疫分析法(ELISA)检测血清IL-2的变化。结果:与正常对照组脾脏指数(0.38±0.03)%比较,衰老模型小鼠脾脏指数(0.25±0.05)%降低,脾组织结构退化明显,IL-2水平(0.90±0.17)μg.L-1明显降低(P<0.05);用药后,延衰合剂高剂量组小鼠脾组织结构明显改善,脾脏指数(0.37±0.04)%增加,IL-2水平(2.47±0.77)μg.L-1增加(P<0.05)。YSM高剂量组作用优于补肾益寿胶囊组及YSM低、中剂量组(P<0.05)。结论:YSM可改善衰老小鼠脾脏病理结构,抑制IL-2水平的下降,提高免疫功能,可能具有良好的延缓衰老的作用。
Objective： To study the effect of Yanshuai mixture（YSM） on the spleen tissue structure and serum interleukin 2（IL-2） levels in D-galactose induced sub-acute aging mice.Method： The aging mice were established by D-galactose（120 mg·kg-1·d-1） and administered continuously with YSM for 8 weeks.The spleen index and changes in spleen endoscopic biopsy morphology were observed,IL-2 levels in serum were detected using enzyme-linked immunosorbent assay（ELISA）.Result： The spleen index of aging model mice was decreased（0.25±0.05）%,histopathological changes in spleen were degraded significantly,and IL-2 level（0.90±0.17）μg·L-1 was significantly reduced compared with those in normal control group（P0.05）.After the treatment with YSM,spleen histomorphological changes were ameliorated,the spleen index of high-dose YSM mice（0.37±0.04） was increased and level of IL-2 in the serum（2.47±0.77）μg·L-1 was increased（P0.05）.High-dose YSM group was superior to Bushen Yishou capsule（BSYS） group,low and middle-dose YSM group（P0.05）.Conclusion： YSM can improve the spleen histopathological changes,inhibit the decline of IL-2 level in serum,and improve immune function,so it may have a good anti-aging effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第16期188-191,共4页
Chinese Journal of Experimental Traditional Medical Formulae
