摘要
采用离子交联法和冷冻干燥法制备强力霉素壳聚糖纳米粒冻干粉,并以包封率为评价指标,正交试验优化处方与工艺,用透射电镜观测其形态,纳米粒度及电位分析仪测定其粒径大小、分布和电位,并考察其热、光和高湿稳定性。结果显示,按壳聚糖浓度为2.5 mg/mL、三聚磷酸钠浓度为1.2 mg/mL、强力霉素用量为8.0 mg、pH值为5.5的处方制备的强力霉素壳聚糖纳米粒冻干粉表面蓬松,复溶性良好,电镜下观测发现纳米粒形态圆整、粒径大小均匀,平均粒径为(126.3±17.8)nm,平均包封率为(56.52±2.1)%,平均载药量为(16.83±0.27)%。与原料药相比,10 d强力霉素壳聚糖纳米粒冻干粉的(4500±500)Lx强光降解率减少22.97%,40、60℃热降解率分别减少18.72%和31.41%,75%、92.5%高湿降解率分别减少20.45%和26.90%。强力霉素壳聚糖纳米粒冻干粉制备工艺切实可行,稳定性良好。
Doxycycline Chitosan Nanoparticles（DC-CS-NPs） freeze dried powder was prepared by using freeze drying and ionic gelation method.The preparation was optimized by orthogonal experimental design with encapsulation efficiency as evaluation index.The particle morphology,size and potential were determined respectively by transmission electron microscopy,particle and potentiometric analyzer.The ultraviolet,thermal and humid stabilities were measured at the same time.The optimal preparation process of DC-CS-NPs was 2.5 mg/mL chitosan solution,1.2 mg/mL sodium tripolyphosphate solution,8 mg doxycycline and pH 5.5.The DC-CS-NPs freeze dried powder had a fluffy surface and good solubility.The DC-CS-NPs were discrete and uniform spheres under transmission electron microscopy with a mean particle size of（126.3±17.8）nm.The encapsulation efficiency and drug loading of DC-CS-NPs were（56.52±2.1）% and（16.83±0.27）%,respectively.Compared to DC raw material drug,the ultraviolet degradation of DC-CS-NPs freeze-dried powder in 10 d under bright lights of（4500±500）Lx decreased by 22.97%,and the thermal degradations in 10 d decreased by 18.72% at 40 ℃ and 31.41% at 60 ℃,while the humid degradations in 10 d decreased by 20.45% at 75% and 26.90% at 92.5%.The DC-CS-NPs freeze dried powder,with a feasible process of preparation,has a characteristic of good stabilities.
出处
《淡水渔业》
CSCD
北大核心
2011年第3期55-60,共6页
Freshwater Fisheries
基金
农业部公益性行业科研专项(200803013)
关键词
强力霉素
壳聚糖
纳米粒
冻干粉
稳定性
doxycycline
chitosan
nanoparticles
freeze-dried powder
stability
