摘要
为确定四川省部分地区黄鳝(Monopterus albus)出血病病原的种类及耐药情况,从患出血病濒死的黄鳝肝脏、肾脏、脾脏等组织取样进行常规细菌学检查,对分离菌株进行形态观察、生理生化特性和16S rDNA基因分析,并进行动物回归试验及药敏试验。结果分离得到14株病原菌株,经形态特征、理化特性及16S rDNA序列分析,判定14株细菌均为嗜水气单胞菌(Aeromonas hydrophila)。14株病原菌株对泥鳅(Misgurnus anguillicauda-tus)、云斑(Amiurus nebulosus)和小白鼠均有较强的致病力;对萘啶酸、诺氟沙星、四环素、多西环素高度敏感;对磺胺异恶唑、羧苄西林、氨苄西林耐药;对亚胺硫霉素、多粘菌素B呈现出不同的敏感性。研究结果表明嗜水气单胞菌为引起当地黄鳝出血病的主要病原菌。
This experiment was conducted to clarify pathogenic species of hemorrhagic septicemia disease and drug resistance in rice field eels（Monopterus albus） from Sichuan province.Pathogenic bacteria from liver,kidney and spleen of diseased and dying rice field eels were examined using conventional methods and isolated.Morphological,physiological and biochemical characteristics of the isolated bacteria were investigated.Moreover,the 16S rDNA gene was sequenced and analyzed.Meanwhile,animal regression experiment and antibiotic sensitivity test about the isolated bacteria were all conducted.Fourteen bacteria strains were isolated from the rice field eels.These isolated bacteria strains were identified as Aeromonas hydrophila by morphological,physiological and biochemical characteristics,and by the 16S rDNA gene sequence analysis.The isolated fourteen bacteria strains had strong pathogenicity to Misgurnus anguillicaudatus,Amiurus nebulosus and mice.The antibiotic susceptibility test revealed that the A.hydrophila strain was highly sensitive to nalidixic acid,norfloxacin,etracycline,and doxycycline,and resistant to sulfafurazole,carbenicillin,and ampicillin suppositories.There were different levels of sensitivity to imipenem and aerosporin.The results suggested that the A.hydrophila strain was the main bacterial pathogen leading to hemorrhagic septicemia disease among rice field eels in Sichuan province.
出处
《淡水渔业》
CSCD
北大核心
2011年第3期72-76,共5页
Freshwater Fisheries
基金
教育部<长江学者和创新团队发展计划>创新团队项目(IRTO848)
