摘要
采用人工气候箱,研究了热激萌动种子(42℃、2h)、脱落酸(ABA)(1×10-5mol/L)浸种(6 h)、2叶期叶面喷施和根部灌施,对棉苗抵御(模拟)高温干旱逆境能力的影响。结果表明,在高温干旱期间,与对照相比,所有处理都合成了差异蛋白质,但ABA浸种所合成的热激蛋白(HSP)更丰富。另外,所有处理都显著提高了棉苗超氧化物歧化酶(SOD)活性;ABA浸种、根部灌施处理和热激处理还显著提高了棉苗过氧化物酶(POD)活性;ABA叶面喷施处理和热激处理显著提高了棉苗过氧化氢酶(CAT)活性。ABA叶面喷施处理和浸种处理降低了棉苗丙二醛(MDA)含量。
In growth chamber,the effect of heat shock on germination seeds（42℃,2h）,ABA（1×10-5mol/L） soaking seeds（6 h）,irrigating roots（5 mL 1×10-5mol/L ABA per plant） and spraying on leaf（5mL 1×10-5mol/L ABA per plant） at 2-leaf stage on cotton seedlings were studied under simulated high temperature（35 ℃） and water deficit（4 d of drying） condition.The results showed that,during the high-temperature and drought period,cotton plants treated produced HSP,and the HSPs were more abundant in the treatment of soaking seed with ABA.All treatments increased significantly the SOD activity of seedlings.Heat shock,soaking seed and root irrigation with ABA all enhanced the activity of POD.Heat shock and ABA leaf spraying also increased the activity of CAT.Besides,soaking seed and leaf spraying with ABA lowered the content of MDA.
出处
《湖北农业科学》
北大核心
2011年第14期2828-2831,共4页
Hubei Agricultural Sciences
基金
农业部行业计划项目(3-5)
湖北省农业科技创新中心资助项目(2007-620-001-03)
关键词
棉花
脱落酸
热激
抗氧化酶
cotton
abscisic acid（ABA）
heat shock
antioxidant enzyme
