摘要
目的在慢性氟中毒实验中研究不同浓度氟对大鼠骨密度的影响。方法 Wistar大鼠40只,雌雄各半,随机分为四组,其中三组高、中、低氟染毒组分别饮用氟(F-)浓度为200、100、50 mg/L的含氟水,一组为对照组,饮用蒸馏水。饲养2个月、4个月和6个月时使用双能X线骨密度仪测量大鼠双侧股骨骨密度(BMD)。结果染毒2个月时各组骨密度比较:高氟组、中氟组>低氟组、空白组(P<0.05),高氟组≈中氟组,低氟组≈空白组(P>0.05);染毒4个月时骨密度比较:高氟、中氟和低氟组>空白组(P<0.05),高氟组≈中氟组≈低氟组(P>0.05);染毒6个月后骨密度比较:高氟组≠中氟组≠低氟组≠空白组(P<0.05),且低氟组>中氟组>空白组>高氟组(P<0.05)。同组自身前后骨密度比较即各组组内骨密度比较,高氟组:2月、4月>6月(P<0.05),2月≈4月(P>0.05);中氟组:2月、4月<6月(P<0.05),2月≈4月(P>0.05);低氟组:2月<4月、6月(P<0.05),4月≈6月(P>0.05);对照组2、4、6三个月时的骨密度比较均无显著差异(P>0.05)。结论长期小剂量氟和短期大剂量氟染毒可引起大鼠骨密度增高和骨质硬化表现,而长期大剂量氟染毒则导致大鼠骨密度降低出现骨质疏松改变。
Objective To study the effect of different doses of fluorine ion on bone mineral density （BMD） in a rat chronic fluorosis model. Methods Forty Wistar rats were randomly divided into 4 groups and each group had 5 male and 5 female rats. Rats in the control group was given distilled water, and in other groups of the fluoride was administered with water containing 200 mg/L （high-dose） , 100 mg/L （middle-dose） , or 50 mg/L （low-dose） of fluorine ion （F-respectively）. BMD of both femurs was examined with dual-energy X-ray densitometry after 2-month, 4-month, and 6-month feeding. Results The comparison of BMD after 2-month exposure to fluorine showed that BMD in high-dose group and middle-dose group was higher than that in low-dose group and control group （P 〈 0.05 ）. BMD was not different between high-dose group and middle-dose group, and between low-dose group and control group （P 〉0. 05）. The comparison of BMD after 4-month exposure to fluorine showed that BMD in high-dose, middle-dose, and low-dose group was higher than that in control group （P 〈 0.05）. BMD was not different among high-dose, middle-dose, and low-dose group （P 〉0. 05）. The comparison of BMD after 6-month exposure to fluorine showed that BMD was different among the groups （ P 〈 0. 05 ）. The highest BMD was in low-dose group, then in middle-dose group, then in control group, and the lowest was in high-dose group （P 〈 0. 05 ）. The comparison of the BMD within each dose group showed that in high-dose group, BMD was higher in 2-month and 4-month exposure than in 6-month exposure （P 〈 0.05 ）, but was not different between 2-month and 4-month exposure （ P 〉 0.05）. In middle-dose group, BMD was lower in 2-month and 4-month exposure than in 6- month exposure （ P 〈 0.05） , but was not different between 2-month and 4-month exposure （ P 〉 0. 05 ）. In low-dose group, BMD was lower in 2-month exposure than in 4-month and 6-month exposure （ P 〈 0.05 ） , but was not different between 4-month and 6-month exposure （P 〉 0. 05）. In control group, no significant difference was found among BMD of 2-month, 4-month, and 6-month exposure （P 〉 0.05 ）. Conclusion Long-term and low-dose or short-term and high-dose use of fluoride can cause increased BMD and osteoselerosis in rats. Long-term and high-dose of fluoride can lead to decreased BMD and osteoporosis.
出处
《中国骨质疏松杂志》
CAS
CSCD
2011年第8期674-676,共3页
Chinese Journal of Osteoporosis
关键词
大鼠
氟中毒
骨密度
骨质疏松
骨质硬化
Rat
Fluorosis
Bone minal density
Osteoporosis
Osteosclerosis
