摘要
目的观察雌性小鼠亚急性接触柴油机尾气颗粒物（diesel exhaust particles，DEP）致卵和卵裂球损伤以及复方当归注射液对损伤的干预效果。方法210只21dICR雌鼠随机分为对照组（A组）、DEP组（B组）、DEP＋小剂量组（C组）、DEP＋中剂量组（D组）和DEP＋大剂量组（E组）（各42只），分别向小鼠咽后壁接种12．0μg／μlDEP混悬液（B～E组）或载液（PBS，A组）各30μl，每3d重复接种1次，共4次。末次DEP接触后3d处死动物。C、D、E组首次接种日至处死前1d，每日分别经腹腔给予复方当归注射液相当于生药75、150和300mg各1次，连续给药12次。观察指标包括小鼠一般情况、体重；卵巢重量、卵巢重量／体重比值、卵巢组织超氧化物歧化酶（superoxide dismutase，SOD）活性、还原型谷胱甘肽（reduced glutathione，GSH）和丙二醛（malonaldehyde，MDA）含量；卯母细胞的存活率、胚泡破裂比率、第一极体释放率、受精率、线粒体DNA含量和超微结构变化。结果（1）各组小鼠体重差异无统计学意义（P〉0．05）。卵巢重量、卵巢重量／体重比值、卵巢SOD活性和GSH含量B组分别为（1．5±0．6）mg、（7．2±2．5）×10^-5、（192．10±23．67）nU／mgprot和（262．40±31．60）nmol／mgprot，C组分别为（1．7±0．2）mg、（8．9±0．6）×10^-5、（198．92±24．27）nU／mgprot和（271．66±14．58）nmol／mgprot，D组分别为（2．1±0．2）mg、（9．8±1．1）×10^-5、（214．37±27．19）nU／mgprot和（285．93±9．55）nmol／mgprot，低于A组，分别为（3．3±1．5）mg、（15．4±7．3）×10^-5、（292．30±40．03）nU／mgprot和（367．98±24．59）nmol／mgprot（P〈0．05或〈0．01）；E组分别为（3．7±1．1）mg、（18．7±5．4）×10^-5、（279．10±12．63）nU／mgprot和（353．59±10．61）nmol／mgprot，显著高于B组（P〈0．01）；MDA含量B、C、D组分别为（3．88±0．35）nmol／mgprot、（3．62±0．19）nmol／mgprot和（2．63±0．34）nmol／mgprot，显著高于A组的（2．18±0．44）nmol／mgprot（P〈0．05或〈0．01），D、E组[（2．35±0．37nmol／mgprot]显著低于B组（P〈0．01）。（2）观察时间B、C组卵母细胞存活率以及B、C、D组第一极体释放率和24h体外受精率显著低于A组（P〈0．05或P〈0．01），E组显著高于B组（P〈0．05）；各组胚泡破裂比率均为100％。（3）卵母细胞线粒体DNA的拷贝数对数值，与A组比较，C、D、E组显著降低（P〈0．01）；与B组比较，C、D组显著降低而E组显著升高（P〈0．01）。（4）A组卵母细胞结构无明显改变；B、C组卵母细胞许多细胞器退变明显，部分细胞坏死；D组细胞内较多线粒体肿胀、空泡化，E组这些变化范围及程度均减小。结论复方当归注射液对雌性小鼠亚急性接触DEP所致的卵和卵裂球损害有治疗及保护作用。
Objective To investigate the effects of compound radix angelicae sinensis injection on oocyte and segmentation sphere injured by subacute exposure to diesel exhaust particles （DEP） in female mice. Methods Two hundred and ten 21-day-old ICR female mice were randomly divided into 5 groups： the control group （group A）, DEP group （group B）, DEP±low-dose group （group C）, DEp±middle-dose group （group D） and DEP±high-dose group （group E）. There were 42 mice in each group. The mice were inoculated with 30 μ1 DEP suspension at 12.0μg/μl （group B-E） or the same volume of vehicle （PBS, group A） on pharynx posterior wall by sample pipettor beginning at day 21 and repeated every 3 days for 4 times. The mice were sacrificed three days after the last exposure. Compound radix angelicae sinensis injection containing 75 （group C）, 150 （group D） and 300 （group E） grams of crude drug, respectively, which was intraperitoneally administered for each mouse daily from the day of the first DEP inoculation till the day before sacrifice, consecutively for 12 days. The general conditions were observed, and the body weight and ovary/body weight ratio were tested. Superoxide dismutase （SOD） activity, malondialdehyde （MDA） and reduced glutathione （GSH） contents in ovarian tissues were assayed. Rates of survival, germinal vesicle breakdown, extrusion of the first polar body and in-vitro fertilization, and quantity of mitochondrial DNA for oocytes were investigated. Ultrastructural changes of oocytes were observed. Results （1） No significant difference of the body weight was found among all the groups （P〉0.05）. The ovary weight, ovary/body weight ratio, ovary SOD and GSH content were significantly decreased in groups B[（1.5 ± 0.6） mg, （7.2 ± 2.5） ×10^-5 , （192.10±23.67） nU/mg prot and （262.40±31.60） nmol/mg prot], and C [（1.7±0.2） mg, （8.9±0.6）×10^-5, （198.92±24.27） nU/mg prot and （271.66±14.58） nmol/mg prot] and D E（2. 1±0. 2） mg, （9. 8±1.1）×10^-5, （214. 37±27. 19） nU/mg prot and （285. 93±9. 55） nmol/mg prot] as comparing to group A [（3.3±1.5） mg, （15.4±7.3）×10^-5 , （292.30±40.03） nU/mg prot and （367.98± 24.59） nmol/mg prot （P〈0.05 or PG0.01）; and significantly increased in group E E（3. 7±1.1） mg, （18. 7±5.4） ×10^-5, （279. 10±12. 63） nU/mg prot ]and （353. 59±10. 61） nmol/mg prot] comparing to group B （P〈0.01）. MDA content was signi-ficantly increased for groups B, C and D [（3.88±0.35） nmol/mg prot, （3.62±0.19） nmol/mg prot and （2.63 ±0.34） nmol/mg prot] comparing to group A E（2.18±0.44） nmol/mg prot]（P〈0. 05 or 〈0.01, respectively） ; and significantly decreased for group D and E （2. 35±0. 37 nmol/mg prot） comparing to group B （P〈0. 01）. （2） In all observed time points, oocyte survival rate in group B and C, extrusion rate of the first polar body and in-vitro fertilization rate in group B, C and D were significantly lower than in group A （P 〈 0.05 or 〈0.01）, and all those in group E were significantly higher than in group B （P〈0.05）. Rates of germinal vesicle breakdown were 100% in all five groups. （3） Logarithmic values of mitochondrial DNA copy numbers in group C, D and E were significantly lower than in group A; whereas significantly higher in group C and D than in group B （P〈0.01）. （4） Normal appearance for oocytes in group A was seen. In groups B and C, a number of cytoplasmic organelles were dramatically degenerated in part of the oocytes and some necrotic oocytes were seen. Large body of mitochondria in the oocytes swelled and vacuolized in group D, while such changes dincished to a lesser extent and scope in group E. Conclusions Compound radix angelicae sinensis injection exerts a favorable curative and protective effect on oocyte and segmentation sphere injured by subacute DEP exposure in female mice.
出处
《中华围产医学杂志》
CAS
2011年第8期475-482,共8页
Chinese Journal of Perinatal Medicine
基金
南京军区医学科技资助项目（07M093）
福建省科技厅计划重点项目基金（2007Y0017）
美国健康影响研究院基金（4747.RFPA05-3/06-8）
关键词
丹参注射液
复方合剂
车辆排放物
颗粒物
卵母细胞
小鼠
Danshen injection
Drug combinations
Vehicle emissions
Particulate matter
Oocytes
Mice
