摘要
为促进温氏支原体的深入研究,本试验建立了温氏支原体感染动物模型。选择6～8周龄健康雄性昆明种小鼠60只,随机分为A、B、C、D 4组,试验组(A、B、C)分别以不同方式感染温氏支原体,D组为对照组。结果表明,腹腔注射一定剂量高感染强度的牛红细胞悬液,同时注射免疫抑制剂地塞米松组(C组)表现为首现期出现早,且高峰期红细胞感染率高。应用悬滴镜检法和PCR诊断方法对感染小鼠血样进行鉴定,均符合温氏支原体特征。选择对照组小鼠和感染率高、临床症状较为明显的C组小鼠各10只,进行血液生理生化指标测定(RBC、WBC、Hb、TP、G、ALT、AST),结果呈现出与牛感染温氏支原体相同的规律。
This study is to establish an animal model for Eperythrozoon wenyoni infection. Sixty Kunming mice of 6 to 8 weeks old were selected, and divided into four groups randomly： A, B, C and D groups. The experimental groups （A, B and C） were infected with E- wenyoni at the different ways. The results showed that the appearance period was earlier, and the infective rate of erythrocyte （RBC） was higher among peak period in group C, in which bovine RBC suspension with higher infective intensity was injected and the immunosuppressant dexamethasone was used at same time. The bloods from the infected mice were investigated by microscopical observation and PCR amplification. Ten mice from control group D and infective group C were selected respectively, and their hematological indexes, including RBC, WBC, Hb, TP, G, ALT and AST, were determined. The results showed the same law as that in the cattle infected with E. wenyoni.
出处
《畜牧与兽医》
北大核心
2011年第8期22-25,共4页
Animal Husbandry & Veterinary Medicine
基金
河北省畜牧局课题(2006-11)
