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基于纳米银增强的酶基化学发光生物传感器测定H_2O_2 被引量:4

Chemiluminescence biosensor based on silver nanoparticle-enhanced activity of horseradish peroxidase for determination of H_2O_2
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摘要 利用溶胶-凝胶法将辣根过氧化物酶(HRP)和纳米银(Ag NPs)颗粒共固定,制备了一种新的流通式化学发光过氧化氢传感器.结果表明,在纳米银的存在下,传感器的响应增强了10倍,线性范围为7×10-5~1×10-3mol/L,检出限为2.4×10-6mol/L,标准偏差为3.2%(4×10-4mol/L H2O2,n=9). A new chemiluminescence(CL) flow-through biosensor for determination of hydrogen peroxide was developed by immobilizing horseradish peroxidase(HRP) and Ag nanoparticles with the Sol-gel method on the flow CL cell.In the presence of Ag nanoparticles,the respond of the biosensor is enhanced about 10-fold.The calibration graph is linear in the range of 7×10-5 to 1×10-3 mol/L,and the detection limit is 2.4×10-6 mol/L.The relative standard deviation is 3.2%(4×10-4 mol/L H2O2,n=9).
出处 《陕西师范大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第4期51-54,共4页 Journal of Shaanxi Normal University:Natural Science Edition
基金 国家自然科学基金资助项目(20405009)
关键词 生物传感器 辣根过氧化物酶 纳米银 化学发光 biosensor HRP Ag nanoparticles chemiluminescence
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参考文献12

  • 1Ionescu R E, Cosnier S, Marks R S. Protease amperometric sensor [J] . Analytical Chemistry, 2006, 78 (18) : 6327-6331.
  • 2Pandey P, Singh S P, Arya S K, et al. Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity [J]. Langmuir, 2007, 23 (6): 3333-3337.
  • 3吕勇军,李佩晋,郭杨龙,王艳芹,卢冠忠.酶在有序介孔材料上的固定化[J].化学进展,2008,20(7):1172-1179. 被引量:17
  • 4高盐生,付秀军,吴定宏.生物传感器中酶的固定化方法[J].山东化工,2008,37(4):21-22. 被引量:3
  • 5Chu Xia, Duan Daxue, Shen Guoli, et al. Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode [J]. Talanta, 2007, 71 (5): 2040-2047.
  • 6Choi M M F. Application of a long shelf-life biosensor for the analysis of L-lactate in dairy products and serum samples [J]. Food Chemistry, 2005, 92 (3): 575-581.
  • 7Shen C, Kostic N M. Kinetics of photoinduced electrontransfer reactions within Sol-gel silica glass doped with zinc eytochrome c. study of electrostatic effects in confined liquids [J]. Journal of the American Chemical Society, 1997, 119(6), 1304-1312.
  • 8Shi Jinjun, Zhu Yongfa, Zhang Xinrong, et al. Recent developments in nanomaterial optical sensors[J].Trends Analytical Chemistry, 2004, 23(5): 351-360.
  • 9霍书豪,许敬亮,张猛,庄新姝,徐惠娟,亓伟,袁振宏,杨秀山.Fe3O4纳米颗粒固定化纤维素酶的酶学特性研究[J].可再生能源,2009,27(6):33-35. 被引量:3
  • 10Di Junwei, Shen Chunping, Peng Shaohua, et al. A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanopartides embedded in silica Sol-gel network on gold modified electrode[J].Analytical Chimistry Acta, 2005, 553 (1/2) : 196-200.

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