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胃癌微卫星不稳定性与移码突变的关系 被引量:9

Microsatellite instability and frameshift mutation in gastric carcinoma
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摘要 目的 研究胃癌微卫星不稳定( MSI) 与移码突变的关系。方法 采用PCR为基础的方法检测了44 例胃癌8 个微卫星位点和hMSH6 、BAX和TGFβRⅡ移码突变。结果 胃癌MSI的检出率为25.0 % (11/44 例) 。6 例(13 .6% ) 为高MSI(4 个以上位点),5 例(11 .4 % )为低MSI(1 ~3 个位点)。肠型胃癌高MSI的检出率显著高于弥漫型胃癌( P< 0.05) 。44 例胃癌中检出TGFβRⅡ移码突变4 例,BAX 和hMSH6 基因移码突变各1 例。以上移码突变均发生于高MSI组,而低MSI和MSI阴性组未见有移码突变。结论 高MSI胃癌有单核苷酸移码突变倾向。 Objective To study the relationship between microsatellite instability (MSI) and frameshift mutations of hMSH6, BAX and TGFβ RⅡ. Methods Eight microsatellite loci and frameshift mutations of hMSH6, BAX and TGFβ RⅡ were analyzed by polymerase chain reaction (PCR) based methods. Results MSI was detected in 25.0% (11/44) of gastric cancer. Six (13.6%) gastric carcinomas were high MSI (Four Loci or morc) and five (11.4%) were low MSI (one to three loci). The frequency of MSI high proved to be significantly higher in intestinal type than in diffuse carcinomas ( P <0.05). Of the 44 gastric carcinomas, frameshift mutations were detected in 4 for TGFβ RⅡ, 1 for BAX and 1 for hMSH6. All frameshift mutations were detected in gastric carcinomas with high MSI, none was detected in those with low MSI or MSI negative. Conclusions Gastric cancers with high MSI exihibits a tendency to accumulate frameshift mutations in simple repeated nucleotide sequence.
出处 《中华消化杂志》 CAS CSCD 北大核心 1999年第6期385-387,共3页 Chinese Journal of Digestion
基金 国家自然科学基金
关键词 胃肿瘤 微卫星不稳定性 移码突变 Stomach ncoplasms Microsatellite instability Frameshift mutation
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参考文献3

  • 1Yin J,Hum Mutat,1997年,10期,474页
  • 2Chung Y J,Oncogene,1997年,15卷,1719页
  • 3周晓东,中华医学杂志,1997年,27期,850页

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