摘要
目的研究天疱疮抗体结合表皮细胞间抗原在超微结构水平的部位。方法采用LRWhite树脂为包埋剂，用金标记包埋后直接和间接免疫电镜技术，观察天疱疮患者皮损中IgG的沉积部位和患者血清中IgG型自身抗体结合正常人皮肤的部位。结果寻常型天疱疮和落叶型天疱疮的直接和间接免疫电镜均在表皮细胞间的桥粒部位见金颗粒沉积，在非桥粒部位的角质形成细胞间未见金颗粒沉积。结论寻常型天疱疮和落叶型天疱疮的靶抗原均是桥粒成分，均位于桥粒复合体上。
Objective To explore the location of binding sites of autoantibodies in patients with pemphigus. Methods The direct and indirect immunogold labeling electron microscopic techniques were used to detect the ultrastructural location of the immunoglobulin G in the skin lesions of five patients(pemphigus vulgaris 4, pemphigus foliaceus 1), and location of binding sites of the circulating autoantibodies with normal human skin in the sera of eight patients(pemphigus vulgaris 6, pemphigus foliaceus 2), using LR White resin as embedding reagent. Results Binding of gold labeling anti-human IgC was observed predominantly on desmo-somal complex, but not on non-desmosomal cell surface of keratinocytes. Conclusion The target antigens of pemphigus vulgaris and pemphigus foliaceus are components of desmosome, and these antigens are located on the desmosomal complex.[
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1999年第6期381-382,共2页
Chinese Journal of Dermatology
基金
安徽省自然科学基金!97424005
