摘要
目的 研究单唾液酸四已糖神经节苷脂（ＧＭ１）对大鼠全脑缺血再灌注中钙平衡紊乱、氧自由基代谢异常以及病理损伤的影响。方法 运用大鼠全脑缺血再灌注模型（４ＶＯ），观察假手术组、脑缺血３０ 分钟再灌注６０ 分钟生理盐水（ＮＳ）处理组（ＮＳ组）和缺血３０ 分钟再灌注６０ 分钟ＧＭ１ 处理组（ＧＭ１ 组）的脑海马组织线粒体钙（ＭＣａ）、钙调素（ＣａＭ）、丙二醛（ＭＤＡ）及海马ＣＡ１ 区病理改变。结果 ＮＳ组海马组织ＭＣａ、ＣａＭ、ＭＤＡ含量显著升高（Ｐ＜ ０．０１），海马ＣＡ１ 区神经元呈较严重缺血损伤性改变，而ＧＭ１ 组较ＮＳ组生化和病理变化明显改善。结论 ＧＭ１ 能改善脑缺血再灌注中钙平衡紊乱和氧自由基代谢异常，减轻脑缺血再灌注病理损伤。
Objective To study the effect of GM 1 to the abnormal of calcium balance,oxygen free radicals metabolism and pathologic changes on rat global cerebral ischemic reperfusion damage.Methods With rat global cerebral ischemia and reperfusion model (4VO), we measured the rat hippocampic mitochondria calcium (MCa),calmodulin (CaM),malondiadehyde (MDA) contents and the pathologic changes of hippocampic CA 1 district in the sham group,cerebral ischemia for 30 min and reperfusion for 60 min group treated by normal sodium (NS) and GM 1 group,cerebral ischemia for 30 min and reperfusion for 60 min.Results The hippocampic MCa,CaM,MDA contents were significantly higher in NS group (P< 0.01 ),the hippocampic CA 1 district neuron showed severe ischemic damage.The changes of biochemistry and pathology had more remarkable improvement in GM 1 group than NS one.Conclusion GM 1 could improve abnormal of calcium balance,oxygen free radicals metabolism in cerebral ischemic reperfusion damage,and reduced its pathologic injury.
出处
《临床神经病学杂志》
CAS
1999年第6期326-328,共3页
Journal of Clinical Neurology
关键词
线粒体钙
钙调素
脑缺血
再灌注损伤
GM1
Monosidoltetrahexosylganglioside Mitochondria calcium Calmodulin Oxygen free radicals Cerebral ischemic reperfusion damage
