摘要
目的:利用肽羧合剂EDC[1-乙基-3-(3-二甲基氨丙基)-碳二亚胺盐酸盐]将粘附蛋白类八肽(谷氨酸-脯氨酸-精氨酸-甘氨酸-天冬氨酸-天冬酰胺-酪氨酸-精氨酸)耦联到经紫外线辐照活化的小牛松质骨片上,制备出用于骨缺损性疾病的骨修复与重建的组织工程新方法,为口腔医学中牙齿和颌骨缺损性疾病的治疗提供骨移植的新型材料。方法:采用125I-RGD放射性示踪技术、碳二亚胺羧合、紫外线辐照活化的小牛松质骨的羧基及蛋白质染色技术。结果:牢固地将RGD耦联到小牛松质骨上。结论:随着骨组织工程研究的发展和这一新材料的深入研究,将改变骨缺损临床采用羟基磷灰石或骨水泥等材料将缺损部位封闭,从而使其丧失功能的治疗方法,引领骨移植临床应用的到来。
Objective：to use peptide carboxy mixture EDC（1-ethyl-3-（3-2 methyl ammonia isopropyl）-carbon two imine）will adhesion protein class eight peptide（Glu-Pro-Arg-Gly-Asn-Asn-Tyr-Arg RGD） by ultraviolet irradiation coupling to activate the mavericks cancellous bone chip,which is prepared for bone defect sex disease of rehabilitation and reconstruction of bone tissue engineering,especially a new method of oral medicine teeth and jaws defect sexual diseases treatment provides bone grafts new material.Method：using 125I-RGD radioactive tracer technology,carbon two imine carboxy close,the mavericks ultraviolet irradiation activation cancellous bone carboxyl and protein dyeing of technology.Result：firmly coupling to Dallas will RGD cancellous bone.Conclusion：along with the development of tissue engineering research and the new materials of thorough research,will change bone defect clinical use a hydroxyapatite or bone cement materials will defect site sealed dead,so the loss of function treatment.The clinical application of bone graft coming.
出处
《临床口腔医学杂志》
2011年第8期461-463,共3页
Journal of Clinical Stomatology
关键词
骨组织工程
放射性示踪
RGD
bone tissue engineering
radioactive tracwr
RGD
