摘要
目的探讨活化白细胞黏附分子(ALCAM)在卵巢良性、交界性和恶性上皮性肿瘤中的表达及其与卵巢癌的临床病理特征的关系,与癌抗原125(CA125)在卵巢肿瘤中的表达水平的相关性。方法采用免疫组织化学EnVision两步法观察68例卵巢上皮性肿瘤组织中ALCAM、CA125在细胞膜或细胞质中出现染色颗粒的强度,判断其在卵巢良性、交界性、恶性上皮性肿瘤中的表达情况。结果 ALCAM在卵巢良性上皮性肿瘤中低表达(20%),在交界性和恶性上皮性肿瘤中表达增加,分别为52.9%、94.4%;恶性与良性和交界性病变相比较差别有显著性。CA125阳性表达率为良性26.6%、交界性70.6%、恶性94.4%,在交界性和恶性卵巢肿瘤组织的蛋白表达明显高于良性肿瘤中的表达,差异有显著性。ALCAM、CA125表达与卵巢癌患者的年龄、肿瘤大小、病理分级、远处转移等无关。ALCAM在卵巢上皮性肿瘤的阳性表达率为67.6%,CA125阳性表达率为73.5%,两者的表达有相关性。结论 ALCAM在卵巢恶性上皮性肿瘤中表达水平明显上调,提示ALCAM可能在卵巢恶性上皮性肿瘤发生中起重要作用。检测ALCAM的表达可作为预测卵巢恶性上皮性肿瘤发生的一项指标。
Objective To investigate the expression of activated leukocyte cell adhesion molecule（ALCAM/CD166） protein in ovarian benign and malignant border sex,and the relationship between ALCAM expression and clinicopathological features of ovarian cancer.And to exploit the relevancy of the expression level of the tumor markers CA125.Methods Observed the strength of the ALCAM or CA125 dyeing particles in cell membranes and cytoplasm in the tissues of ovarian tumors in 68 patients by using immunohistochemistry（EnVisionTM method）.ALCAM protein and CA125 expression was evaluated in the tissues of ovarian benign.and malignant border sex.Results The ALCAM in ovarian benign epithelial neoplasm low expression is 20%.The expression in the border of sex and malignant epithelial neoplasm increases upto 52.9 per 94.4%,respectively.There is great significance in the comparison between ovarian benign and malignant border sex.The ovarian tumor markers CA125 positive expression rate of benign is 26.6%,of border sex is 70.6,ofmalignant is 94.4%.The protein expression of the malignant border sex is much higher than the expression of the benign.The expression of ALCAN and CA125 has no relationship with the patient＇s age,the size of the tumour,pathological level,and the distant metasis.Positive ALCAM protein expression rate in epithelial ovarian tumor is 67.6% and the ovarian tumor markers CA125 positive expression rate is 73.5%.There does have correlation between these two expressions.Conclusion When the expression level of ALCAM in ovarian malignant epithelial neoplasm is obviously up-regulated,this phenomenon indicates its involvement in the occurrence of ovarian malignant epithelial neoplasm.Detection ALCAM expression can be used as an index of predicting ovarian malignant epithelial neoplasm occurrence.
出处
《福建医科大学学报》
2011年第3期205-207,共3页
Journal of Fujian Medical University
关键词
卵巢肿瘤
活化白细胞黏附分子
CA125抗原
ovarian neoplasms
activated-leukocyte cell adhesion molecule
CA125 antigen
