摘要
目的探讨缺血后处理(IPo)对大鼠小肠缺血-再灌注损伤的保护作用。方法 40只健康无特殊病原体级雄性SD大鼠随机分为4组。S组:假手术组;IR组:缺血-再灌注组,夹闭肠系膜上动脉(SMA)45 min,再灌注2 h;IPr组:缺血预处理组,分离SMA后给予3个循环的缺血及再灌注各2 min处理余同IR组;IPo组:缺血后处理组,夹闭SMA 45 min,给予3个循环的再灌注及缺血各2 min处理后再灌注2 h。再灌注结束时切取小肠行病理分析;ELISA法检测血清IL-1β、IL-6、IL-10及TNF-α;计数肠外组织细菌易位菌落数(CFU/g)。结果病理损伤Chiu′s评分IPr组和IPo组明显低于IR组(P<0.05);与IR组比较,IPr组和IPo组血清IL-1β、IL-2、IL-6及TNF-α明显降低(P<0.05),而IL-10升高(P<0.05);细菌易位S组没有发现菌落,IPr组和IPo组菌落数明显少于IR组。结论缺血后处理可减轻大鼠小肠黏膜缺血-再灌注损伤,抑制促炎性因子释放并能抑制细菌易位。
Objective To investigate protective effect and mechanism of ischemic postconditioning on the rat model of intestinal ischemia-reperfusion. Methods Forty healthy SPF male rats were randomly divided into four groups. The sham group,which underwent a laparotomy and dissection of superior mesenteric artery（SMA） before the peritoneal cavity was closed,the IR group,undergoing mesenteric ischemia（45 minutes） and reperfusion（two hours） ;the IPr group, three cycles of ischemia alternated with three cycles of reperfusion lasting two minutes each were performed before 45 minutes ischemia and 120 minutes reperfusion; and the IPo group,undergoing ischemia（45 minutes） and reperfusion（two hours） （before the beginning of reperfusion,three cycles of reperfusion alternated with three cycles of ischemia lasting two minutes each were performed. At the end of procedure,the intestinal morphometric study was measured by Chiu＇s classification,the TNF-α,IL-1β,IL-6 and IL-10 in serum were measured by ELISA and the colony forming units/g（CFU/g） were counted. Results The degree of pathological impaired of small intestine in IPr and IPo groups were lighter than IR group（P〈0.05）. The IPr and IPo rats had enhancement of IL-10 and suppressed production of serum TNF-α,IL-1β,and IL-6 ,compared to IR group rats（P〈0.05）. The E. coli DH5α was hardly detected in the S group. And the levels of bacterial translocation in the IR group were higher than those of IPr and IPo groups（P〈0.05）. Conclusion The IPo has a positive effect on lessening intestinal mucosa injury,on inhibiting secretion of proinflammatory cytokines, and reducing levels of the bacterial translocation.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第24期2410-2412,共3页
Chongqing medicine
基金
云南省自然科学基金资助项目(2009ZC106M)
关键词
小肠
再灌注损伤
大鼠
缺血后处理
intestine, small
reperfusion injury
rats
ischemic postconditioning
