期刊文献+

强脉冲光对人皮肤MMP-1、MMP-8及TIMP-1表达的影响 被引量:3

Effects of intense pulsed light on matrix metalloproteinases-1,-8 andtissue inhibitor of metalloproteinase-1 from human skin
下载PDF
导出
摘要 目的观察强脉冲光照射后人皮肤中基质金属蛋白酶(matrix metalloproteinases,MMPs)及基质金属蛋白酶组织抑制因子(tissue inhibitor of metalloproteinase,TIMP)的表达,探讨强脉冲光嫩肤的分子生物学机制。方法选择皮肤类型为Ⅲ~Ⅳ型志愿者,年龄大于20岁。制定个体化治疗方案,选择恰当的治疗参数,取560 nm/590 nm的滤光片,单/双/三脉冲的不同脉冲方式,脉宽为2.0~4.0 ms,脉冲延迟25~40 ms,能量密度16~20 J/cm2,治疗间隔3周,治疗次数3~4次,于治疗后2~3周在照射部位及非照射部位各切取1块皮肤样本,约5 mm×5 mm×5 mm大小,免疫组化染色,光镜下观察。结果照射后皮肤MMP-1、MMP-8及TIMP-1均有阳性表达,3周时的MMP-1、MMP-8表达弱于2周时的表达,而3周时的TIMP-1表达与2周时相比差异无统计学意义。非照射部位阴性染色。结论强脉冲光可增强人皮肤中MMP-1、MMP-8、TIMP-1的表达,后3者对皮肤重塑起一定的作用。 Objective To analyze quantitatively the alteration of matrix metalloproteinases-1,-8(MMP-1,MMP-8),tissue inhibitor of metalloproteinase-1(TIMP-1) after radiation with intense pulsed light(IPL) technique in clinic.And to partly explain the treatment mechanism of IPL on sun-damaged human skin and provide a theoretical basis of mechanism,course of therapy and interval time for treatment of IPL on photo aging skin.Methods Volunteers older than 20-years of age whose skin type is Fitzpatrick Ⅲ~Ⅳ were selected.One site of each volunteer was radiated respectively with IPL system at different parameters.Skin specimens were taken at both non-treated and treated locations on days 14 and 21 after IPL irradiation of about the size of 5 mm×5 mm×5 mm.All skin specimens were fixed in formaldehyde fixative buffer.Immunohistochemical staining was performed on each specimen,including observations on changes of MMP-1,MMP-8,TIMP-1 proteins expression after IPL irradiation in order to know their action in dermis remodeling.Results The expressions of MMP-1,MMP-8 and TIMP-1 were detected after treatments.To weeks 2 after treatment.The expressions of MMP-1,MMP-8 became weaker at weeks 3.Expression of TIMP-1 was not significantly different between weeks 2 and 3.There were no expressions in normal skin.Conclusion IPL irradiation could promote the expression of MMP-1,MMP-8 and TIMP-1 in human skin directly.
作者 袁宁 赵俊英
出处 《首都医科大学学报》 CAS 北大核心 2011年第4期464-468,共5页 Journal of Capital Medical University
关键词 强脉冲光 基质金属蛋白酶-1 基质金属蛋白酶-8 基质金属蛋白酶组织抑制因子-1 intense pulsed light matrix metalloproteinase-1 matrix metalloproteinase-8 tissue inhibitor of metalloproteinase-1
  • 相关文献

二级参考文献17

  • 1Bing-Hui Yang, Jing-Lin Xia, Li-Wen Huang, Zhao-You Tang, Ming-Shan Chen, Jin-Qing Li, An-Min Liang, Qin-Guo Mo, Hui-Shan Lu, Chao-Liu Dai, Lu-Nan Yan, Zhi-Jian Yu, Rong-Sheng Rao, Le-Qun Li, Zhi-Xiong Su, Zhuang-Wei Fang Shanghai, China Liver Cancer Institute & Zhongshan Hospital, Fudan University, Shanghai 200032, China,Tumor Hospital, Zhongshan University, Guangzhou 510000, China Tumor Hospital, Guangxi Medical Universi- ty, Nanning 530000, China,Xiehe Hospital, Fujian Medical University, Fuzhou 350000, China,Second Hos- pital, Chinese Medical University, Shenyang 110000, China ,Huaxi Hospital, Sichuan University, Chengdu 610041, China ,Hospital Affiliated to Nantong Medical University, Nantong 226000 , China,Second Hospital, Jiangxi Medical University, Nanchang 330000 , China ,First Hospital Affiliated to Guangxi Medical University, Nanning 530000 , China ,People’s Hospital of Hainan Province, Haikou 570000, China.Changed clinical aspects of primary liver cancer in China during the past 30 years[J].Hepatobiliary & Pancreatic Diseases International,2004,3(2):194-198. 被引量:20
  • 2Negishi K, Wakamatsu S, Kushikata N, et al. Full-face photorejuvenation of photedamaged skin by intense pulsed light with integrated contact cooling: initial experiences in Asian patients. Lasers Surg Med, 2002,30:298-305.
  • 3Patrick H, Bitter J. Noninvasive rejuvenation of photodamaged skin using serial, fun-face Intense pulsed light treatments. Dermatol Surg, 2000,26:835-843.
  • 4Ertrique H, Erick V. Gross and microscopic findings in patients submitted to nonablative full-face resurfacing using intense pulsed light: a preliminary study. Dermatol Surg, 2002,28:651-655.
  • 5Ross E, Sajben F. Nonablative skin remodeling:selective dermal heating with a mid-infrared laser and contact cooling combination. Lasers Surg Med, 2000,26:186-195.
  • 6Seiji Kondo. The roles of cytokines in photoaging. J Dermatol Sci, 2000, 23:30-36.
  • 7James B, Patti O. Long-term clinical results of IPL photorejuvenation.J Cosmet Laser Ther, 2003,5:168-174.
  • 8Victor G, Neil S, Josep L, et al. Effects of intense pulsed light on Sun-damaged human skin, routine, and ultrastructural analysis. Lasers Surg Med, 2002,30:82--85.
  • 9Peter B, Karc C, Agneta T, et al. Facial photo rejuvenation using two different intense pulsed Light wavelength bands. Lasers Surgery Med ,2004,34 : 120-126.
  • 10Alexander CM,Hansell EJ,Behrendtsen O,et al.Expression and function of matrix metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation[].Development.1996

共引文献28

同被引文献39

  • 1王明利,柳大烈,袁强,杜本军.强脉冲光对大鼠皮肤TIMP-1表达的影响[J].中国美容医学,2006,15(2):122-123. 被引量:7
  • 2王明利,柳大烈,袁强,杜本军.强脉冲光对大鼠皮肤MMP-1表达的影响[J].中国美容整形外科杂志,2006,17(5):392-394. 被引量:6
  • 3潘敏,骆丹.基质金属蛋白酶与皮肤病相关性[J].国际皮肤性病学杂志,2007,33(1):53-55. 被引量:3
  • 4Abdou A G, Maraee A H, Saif H F A E. Immunohistochemical Evaluation of COX-1 and COX-2 Expression in Keloid and Hypertrophic Scar[J]. The American Journal of Dermatopathology, 2014, 36(4): 311-317.
  • 5Curran T A, Ghahary A. Evidence of a Role for Fibrocyte and Keratinocyte-like Cells in the Formation of Hypertrophic Scars[J]. Journal of Burn Care & Research, 2013, 34(2): 227-231.
  • 6Aoki M, Miyake K, Ogawa R, et al. siRNA knockdown of tissue inhibitor of metalloproteinase-1 in keloid fibroblasts leads to degradation of collagen type I[J]. Journal of Investigative Dermatology, 2013.
  • 7Li Y, Kilani R T, Rahmani-Neishaboor E, et al. Kynurenine Increases Matrix Metalloproteinase-1 and-3 expression in Cultured Dermal Fibroblasts and Improves Scarring in vivo[J]. Journal of Investigative Dermatology, 2013, 134(3): 643-650.
  • 8Mineo A, Suzuki R, Kuroyanagi Y. Development of an artificial dermis composed of hyaluronic acid and collagen[J]. Journal of Biomaterials Science, Polymer Edition, 2013, 24(6): 726-740.
  • 9Martins V L, Caley M, O’Toole E A. Matrix metalloproteinases and epidermal wound repair[J]. Cell and tissue research, 2013, 351(2): 255-268.
  • 10Kai Kessenbrock. Matrix Metalloproteinases: Regulators of the Tumor Microenvironment[J].Cell,2010,1(141):52-67.

引证文献3

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部