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单克隆抗体人源化技术研究进展 被引量：3

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摘要 单克隆抗体（monoclonal antibody,McAb）是来自单个B淋巴细胞或一个杂交瘤细胞克隆的只识别某一种特定抗原表位（抗原决定簇）的抗体。1975年Kohler和Milstein[1]共同创立了杂交瘤技术,首次获得了人工制备的单克隆抗体。单克隆抗体具有高度的特异性和均一性,且能大量制备,从而为抗体的研究和应用带来了突破,在疾病的预防、
作者 潘阳 王露楠
机构地区 北京协和医学院研究生院 卫生部临床检验中心
出处 《国际检验医学杂志》 CAS 2011年第13期1483-1484,共2页 International Journal of Laboratory Medicine
关键词 细菌噬菌体 人源化抗体 嵌合抗体 CDR移植抗体
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献16

  • 1Kohler G,Milstein C.Continuous culture of fused cells secreting antibody of predefined specificity[J].Nature,1975,256(5517):495-497.
  • 2Demarest SJ,Glaser SM.Antibody therapeutics,antibody engineering,and the merits of protein stability[J].Curr Opin Drug Discov Devel,2008,11(5):675-687.
  • 3Schneider CK.Monoclonal antibodies-regulatory challenges[J].Curr Pharm Biotechnol,2008,9(6):431-438.
  • 4Bebbington C,Yarranton G.Antibodies for the treatment of bacterial infections:current experience and future prospects[J].Curr Opin Biotechnol,2008,19(6):613-619.
  • 5Nakano K,Ishiguro T,Konishi H.Generation of a humanized anti-glypican 3 antibody by CDR grafting and stability optimization[J].Anticancer Drugs,2010,21(10):907-916.
  • 6Almagro JC,Fransson J.Humanization of antibodies[J].Front Biosci,2008,1(13):1619-1633.
  • 7Harding FA,Stickler MM,Razo J,et al.The immunogenicity of humanized and fully human antibodies:residual immunogenicity resides in the CDR regions[J].MAbs,2010,2(3):256-265.
  • 8Kashmiri SV,De Pascalis R,Gonzales NR,et al.SDR grafting-a new approach to antibody humanization[J].J Methods,2005,36(1):25-34.
  • 9Sehlin D,Hedlund M,Lord A,et al.Heavy-chain complementarity-determining regions determine conformation selectivity of anti-αβ antibodies[J].Neurodegener Dis,2011,8(3):117-123.
  • 10Kim KS,Kim HJ,Han BW,et al.Construction of a humanized antibody to hepatitis B surface antigen by specificity-determining residues(SDR)-grafting and de-immunization[J].Biochem Biophys Res Commun,2010,396(2):231-237.

二级参考文献15

共引文献2

同被引文献76

  • 1王臣,温文彦,张春杰.鼠源单克隆抗体人源化研究进展[J].安徽农业科学,2006,34(11):2336-2337. 被引量：4
  • 2K6hler G, Milstein C. Continuous culture of fused cells secreting antibody d predefined specificity[J]. Nature, 1975, 256 (5517): 495-497.
  • 3Demarest S J, Glaser SM. Antibody therapeutics, antibody engineer- ing, and the merits of protein stability[J]. Curr Opin Drug Diseov Bevel, 2008, 11 (5) : 675-687.
  • 4Schneider CK. Monoclonal antibodies--regulatory challenges [J]. Curr Phann Biotechnol, 2008, 9 (6) : 431-438.
  • 5Boulianne GL, Hozumi N, Shulman MJ. Production of functional chimaeric mouse/human antibody[J]. Nature, 1984, 312(5995): 643 -646.
  • 6Siddiqui MZ. Monoelonal antibodies as diagnostics :" an appraisal [J]. Indian J Pharm Sci, 2010, 72( 1 ) : 12-17.
  • 7Nakano K, Ishiguro T, Konishi H, et-al. Generation of a humanized anti-glypican 3 antibody by CDR grafting and stability optimization [J]. Antieaneer Drugs, 2010, 21 (10) : 907-916.
  • 8Harding FA, Stickler MM, Razo J, et al. The immunogenicity of hu- manized and fully human antibodies: residual immunogenicity re- sides in the CDR regions[J]. MAbs, 2010, 2(3) : 256-265.
  • 9Kashmiri SV, De Pascalis R, Gonzales NR, et al. SDR grafting--a new approach to antibody humanization[J]. Methods, 2005, 36 (1) : 25-34.
  • 10Sehlin D, Hedlund M, Lord A, et al. Heavy-chain complementarity-determining regions determine conformation selectivity of anti-otl3 antibodies[J]. Neurodegener Dis, 2011, 8(3): 117-123.

引证文献3

二级引证文献6

国际检验医学杂志
2011年 第13期

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