摘要
目的探讨芦荟大黄素(AE)与5-氟尿嘧啶(5-FU)体外协同作用及对人胃癌细胞增殖及凋亡的影响。方法 MTT法检测药物对细胞增殖抑制作用;光镜观察细胞生长状态,计数细胞分裂指数;电镜观察药物对细胞超微结构的影响;TUNEL法检测细胞凋亡。结果 AE单独应用对细胞的增殖抑制作用弱,与5-FU合用明显抑制细胞生长,呈剂量依赖性;药物作用48 h后,联合用药组细胞分裂指数显著降低,可见大量细胞皱缩,胞膜界限模糊,核膜消失,核质浓缩及凋亡小体形成;TUNEL法分析显示,联合用药组细胞凋亡率为(69.4±4.1)%,与AE组(〔15.7±1.9)%〕、5-FU组〔(33.5±2.8)%〕比较差异显著(P<0.05)。结论 AE与5-FU联合应用能有效抑制胃癌细胞增殖,促进细胞凋亡,二药具有协同增效作用。
Objective To investigate the effect of combination aloe-emodin（AE） with 5-fluorouracil（5-FU） on the proliferation and apoptosis of human gastric cancer cells and the synergy between them.Methods Cells proliferation were evaluated by MTT assay.The morphologic changes of gastric cancer cells were observed and the mitosis index was assessed by inverted microscope.The ultrastructure of gastric cancer cells were observed with the transmission electron microscope（TEM）.TUNEL method was used to detect the apoptosis of gastric cancer cells.Results The inhibitive effect of AE plus 5-FU was significantly stronger than that of AE alone,in a dose-dependent manner.Cells were treated with medicine for 48 h,the mitosis index was decreased significantly,and a large number of cell shrinked,nuclear envelope disappeared.There was nuclear chromatin condensation and apoptotic body formation in the combination treated group.Apoptosis rate in the combination treated group（（69.4±4.1）%） was significant than that of AE group（15.7±1.9） % and 5-FU group（（33.5±2.8）%）（P0.05）.Conclusions The combination of AE and 5-FU shows an effective activity in inhibiting gastric cancer cell proliferation and promoting cell apoptosis and has a synergistic effect between them.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2011年第16期3074-3076,共3页
Chinese Journal of Gerontology
基金
黑龙江省教育厅课题资助项目(No.11541384)
牡丹江市科学技术计划项目(No.G2008s0039)
