摘要
目的建立心可舒胶囊中三七皂苷R1、人参皂苷Rg1的含量测定方法。方法以COSMOSIL5C18-PAQPackedColumn（250mm×4．6mm，5μm）为色谱柱，以乙腈．水（27：73）为流动相，流速为1mL·min-1，ELSD为检测器，漂移管温度：41℃；雾化器（氮气）压力：350kPa。结果三七皂苷R1、人参皂苷Rg1的线性范围分别为0．1232--6．16，0．3272～16．36μg，r值分别为0．9999，0．9997；回收率分别为98．7％，99．2％，RSD分别为2．72％，1．54％。结论该方法简便，准确，灵敏度高，重复性好，可作为心可舒胶囊的含量测定方法。
OBJECTIVE To determine the content of notoginsenoside R1, ginsenoside Rgl in Xinkeshu capsule. METHODS The notoginsenoside R1, ginsenoside Rgl in Xinkeshu capsule were separated on a COSMOSIL 5C18-PAQ Packed column（250 mm ×4.6 mm, 5μm）. The mobile phase was acetonitrile-water（27 ： 73） at the flow rate of 1 mL.min-1. The analytes were detected using ELSD. The drift tube temperature was 41℃, and gas press was 350 kPa. RESULTS The responses of notoginsenoside R1, ginsenoside Rgl were linear in the ranges of 0.123 2-6.16μg and 0.327 2-16.36 p.g, respectively. The average recoveries were 98.7% and 99.2% with RSD 2.72% and 1.54%, respectively. CONCLUSION The described method is simple and accurate. It can be used for the quality control of Xinkeshu capsule.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第8期770-773,共4页
Chinese Journal of Modern Applied Pharmacy
