摘要
以1年生的雷公藤扦插苗为试验材料,对其外施浓度分别为5、10、15、20、25 mg.L-1脱落酸(ABA)溶液,以喷施蒸馏水为对照(CK),并进行连续7 d的低温胁迫处理。研究低温胁迫下ABA对雷公藤幼苗叶片质膜透性、丙二醛(MDA)、保护酶和脯氨酸等生理生化指标的影响。研究表明,喷施浓度为15-20 mg.L-1的ABA能提高雷公藤幼苗的抗冷性,有效地降低叶片相对电导率和MDA的积累,使雷公藤幼苗体内游离脯氨酸的含量升高,减弱了低温胁迫对超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响,当ABA浓度为15 mg.L-1时,其相对电导率和MDA含量最低,分别为45.74%和153.28 nmol.g-1,脯氨酸含量比CK高出72.99μg.g-1;20 mg.L-1ABA处理的幼苗冷害程度显著低于其他处理;经ABA处理的SOD活性随胁迫时间的延长呈现明显的"双峰"趋势,POD活性呈现先上升再下降的"单峰"趋势,当ABA浓度为15、20 mg.L-1时,CAT活性呈现"下降—上升—下降"趋势。
One year-old Tripterygium wilfordii cutting was used as the experiment material.By infliction abscisic acid（ABA） 5,10,15,20,25 mg · L-1 continuously 7 days under low temperature stress,and with the plant spraying distilled water as control treatment,the effects of ABA on membrane permeability,malondialdehyde（MDA） content,protective enzyme activity and proline content were studied in T.wilfordii seedlings leaves.The results showed that spraying 15-20 mg · L-1 ABA improved chilling resistance,effectively reducing relative electrical conductivity and the accumulation of MDA,which increased free proline content of T.wilfordii cuttings,weakened the effect of low temperature stress on superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT） enzyme activities.Relative electrical conductivity and MDA content of 15 mg · L-1 ABA were the lowest,which were 45.74% and 153.28 nmol · g-1 respectively.Proline content raised by 72.99 μg · g-1 compared with the control.Chilling injury degree of 20 mg · L-1 ABA was significantly lower than other treatments.SOD activity with ABA treatment showed obvious double peak tendency with the prolongation of stress time.POD activity showed the single peak tendency first increasing then decreasing.When ABA content was 15 and 20 mg · L-1,CAT activity showed ＂decreasing-increasing-decreasing＂ pattern.
出处
《福建林学院学报》
CSCD
北大核心
2011年第3期198-202,共5页
Journal of Fujian College of Forestry
基金
国家科技支撑计划项目(2009BAI73B00)
福建省科技重大专项基金资助项目(2004YZ02-05)
福建省中药材GAP工程技术研究中心资助项目(2008Y2001)
关键词
雷公藤
低温
脱落酸
生理生化
抗冷性
Tripterygium wilfordii
low temperature
ABA
physiology and biochemistry
resistance to cold
