摘要
建立以2，3，4，6-四乙酰基-β—D-吡喃葡萄糖基异硫氰酸酯（GITC）为手性衍生化试剂测定血浆中普萘洛尔对映体浓度的反相高效液相色谱方法。血浆样品经甲醇除蛋白。采用C18色谱柱（4．61mm×250mm，5μm，Bnentnach），流动相为甲醇-20mmol／LKH2P04的水溶液（75：25）；流速ImL／min；检测波长220nm。结果：S（-）-普萘洛尔和尺（＋）-普萘洛尔检测浓度在1．00-277．78pg／mL浓度范围内与色谱峰面积有良好的线性关系。日内、日间精密度均小于5％，回收率分别为98．95％和105．76％，RSD均小于7．13％。结论：本方法灵敏度高，重现性可用于血浆中普萘洛尔对映体的分离测定。
The purpose is to establish a RP- HPLC method with the chiral derivatization reagent 2,3,4,6 - tetra- D- acety-β-D - glucopy- ranosyl- isothiocyanate for separating and detemaining the propranolol enantiomers in plasma. The proteill in plasma samples were was removed with methanol The RP- HPLC separation of propranolol enantiomers was carried out on a C18 column（4. 6 mm× 250 mm, 5 μm. The mobile phase consisted of methanol - 20mmol/LKH2PO4 and aqueous solution（75 ： 25）. The flow rate was lml/min. The detection wavelength was 220 nm. The linear range was 1.00-277. 78μg/ml. The intra- and inter- day precision was both less than 5%. The recovery was 98. 95%o and 105. 76%, respectively and the RSD was less than 7. 13% The method is sensitive and was a good repeatability for the separation and determination of propranolol enantionmers in plasma.
出处
《药物生物技术》
CAS
CSCD
2011年第4期336-338,共3页
Pharmaceutical Biotechnology
