摘要
采用玻片浸渍法测定东莨菪素对朱砂叶螨的触杀毒力,观察东莨菪素对朱砂叶螨的致毒症状,并测定其对朱砂叶螨神经系统靶标酶(乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶)活性的影响。结果表明,东莨菪素对朱砂叶螨24 h的触杀LC50值为1.24 mg/mL;中毒试螨表现出神经毒剂的致毒症状,如兴奋、痉挛等。在活体和离体试验中,东莨菪素均显著抑制试螨乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性。研究还发现东莨菪素会引起试螨乙酰胆碱含量增加,可能是东莨菪素显著抑制试螨乙酰胆碱酯酶活性引起。据此推测东莨菪素对朱砂叶螨可能有神经毒性。
In this study,the contact toxicity of scopoletin to Tetranychus cinnabarinus was tested using slide dip method,and dynamic toxicosis symptoms of scopoletin-exposed mites were also detailedly observed.The results showed that the contact toxicity LC50 value of scopoletin to T.cinnabarinus was 1.24 mg/mL at 24th h post-treatment.Some typical neurotoxic symptoms such as excitement and convulsions were observed in scopoletin-exposed mites.Subsequently,the changes of the in vitro and ex vitro activities of AChE,MAO,Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase after exposed to scopoletin were assayed.Both the in vivo and ex vivo experiments showed that scopoletin can significantly inhibit the activities of AChE,MAO,Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase,and increase the content of ACh simultaneously.The results leading us to believe that scopoletin may be a neurotoxin
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第8期68-71,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金(30872029)
北京市自然科学基金重点项目(6071001)
北京市自然科学基金(6092007)
北京市教委重点项目(KZ201010020016)
北京市教委平台建设项目
北京市属高校人才强教深化计划项目(PHR20090516
PHR200906134)
关键词
东莨菪素
朱砂叶螨
触杀毒力
酶活性
scopoletin
Tetranychus cinnabarinus
acaricidal activity
enzyme activity
