期刊文献+

Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis 被引量:4

Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis
下载PDF
导出
摘要 The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes. The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes.
出处 《Chinese Science Bulletin》 SCIE EI CAS 2011年第27期2916-2921,共6页
基金 supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01) the National Natural Science Foundation of China (30830015)
关键词 小分子RNA 生物信息学 增殖 分析表征 石莼 测序 高通量 MIRNA Ulva prolifera, small RNA, microRNA, Solexa, bioinformatics
  • 相关文献

参考文献25

  • 1Zeng C K, Zhang D R, Zhang J P, et al. Flora of Chinese Economic Seaweeds. Beijing: Science Press, 1962.
  • 2Hayden H, Blomster J, Maggs C, et al. Linnaeus was right all along: Ulva and enteromorpha are not distinct genera. Eur J Phycol, 2003, 38:277-294.
  • 3Gao S, Chen X Y, Yi Q Q, et al. A strategy for the proliferation of ulva prolifera, main causative species of green tides, with formation of sporangia by fragmentation. PLoS One, 2010, 5:e8571.
  • 4Fletcher R. The Occurrence of "Green Tides"-A review. In: Schramm W, Nienhuis P H, eds. Ecological Studies, Vol. 123. Marine Benthic Vegetation. Recent Changes and the Effects of Eutrophication. Berlin: Springer, 1996.7--43.
  • 5Lau N, Lim L, Weinstein E, et al. An abundant class of tiny rnas with probable regulatory roles in caenorhabditis elegans. Science, 2001, 294:858.
  • 6Lee R C, Ambros V. An extensive class of small rnas in caenorhabditis elegans. Science, 2001, 294:862-864.
  • 7Lagos-quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed rnas. Science, 2001, 294:853.
  • 8Bartel D P. Micrornas genomics, biogenesis, mechanism, and function. Cell, 2004, 116:281-297.
  • 9Reinhart B J, Weinstein E G, Rhoades M W, et al. Micrornas in plants. United State: Patent Aplication Publication. 2004.
  • 10Millar A, Waterhouse P. Plant and animal micrornas: Similarities and differences. Funct Integrat Genomics, 2005, 5:129-135.

同被引文献3

引证文献4

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部