摘要
目的:建立以高效液相色谱法同时测定柴芍丸中芍药苷、栀子苷及丹皮酚含量的方法。方法:色谱柱为InertsilODS-SP(4.6 mm×150 mm,5μm),流动相乙腈-水梯度洗脱,检测波长230 nm,流速1.0 mL.min-1。结果:芍药苷在0.494 4~12.36μg(r=1)线性关系良好,平均回收率为98.77%,RSD 2.1%;栀子苷在0.492 2~12.305μg(r=0.999 9)线性关系良好,平均回收率101.64%,RSD 1.9%;丹皮酚在0.260 2~6.505μg(,r=0.999 9)线性关系良好,平均回收率100.92%,RSD1.7%。结论:方法简便、快速、重复性好,可用于柴芍丸的质量控制。
Objective:To establish an HPLC method for the simultaneous determination of paeoniflorin,geniposide and paeonol in Chaishao Pills.Method: Paeoniflorin,geniposide and paeonol were separated on Inertsil ODS-SP(4.6 mm×150 mm,5 μm) column and detected at 230 nm.The mobile phase was acetonitrile-water with gradient elution.The flow rate was 1.0 mL·min-1.Result: Paeoniflorin,geniposide and paeonol were linear within the range of 0.494 4-12.36 μg(r=1),0.492 2-12.305 μg(r=0.999 9) and 0.260 2-6.505 μg,(r=0.999 9) respectively.The average recovery was 98.77%(2.1%),101.64%(1.9%) and 100.92%(1.7%).Conclusion: The method is simple,reproducible,and it can be used for the quality control of Chaishao pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期72-74,共3页
Chinese Journal of Experimental Traditional Medical Formulae