摘要
目的:探讨外源生长素对黄芩悬浮细胞有效成分和内源激素含量的影响。方法:将稳定继代的黄芩愈伤组织在含有不同浓度NAA或KT的MS液体培养基悬浮震荡培养,光照16 h黑暗8 h进行培养18 d后,采用HPLC的方法,测定黄芩苷的含量,采用酶联免疫吸附法测定内源激素的含量,以黑暗条件为对照。结果:①与对照组相比,光照和黑暗条件下分别加入0.2 mg.L-1NAA后,黄芩苷含量显著降低,分别为未加入NAA时的2.56%,3.36%。②黑暗条件下,加入外源NAA后,ZR含量显著降低,但IAA,ABA和GAs含量变化不明显。③0.2 mg.L-1 NAA和不同浓度KT的协同作用对黄芩苷的含量呈现先增长后降低的趋势。其中1.0 mg.L-1的KT作用下黄芩苷含量最高,且黑暗条件下显著高于光照条件,达到2.13μg.L-1。结论:外源生长素不利于黄芩有效成分黄芩苷的积累,0.2 mg.L-1 NAA+1.0 mg.L-1 KT的组合对黄芩苷的积累量具有显著的促进作用。
Objective：To study the effects of auxin on the active compounds and endogenous hormones in Scutellaria baicalensis suspension cell cultures.Method： The subculture scutellariae callus were cultivated in MS liquid medium containing various concentration of NAA or KT on a gyratory shaker with a 16-h photoperiod.After 18 d,the contents of baicalin and endogenous hormones were determined with HPLC and enzyme-linked immunosorbent assay,respectively.Darkness condition was as control.Result：（1） After 0.2 mg·L-1 NAA treatment,the content of baicalin was 2.56% and 3.36% lower than of untreatment,respectively.（2） After NAA treatment on the dark conditions,the content of ZR was significantly reduced.（3） The synergistic effect of 0.2 mg·L-1 NAA with different concentration of KT on the content of baicalin presented the trend of rising then declined.And after 1.0 mg·L-1 KT treatment,the content of baicalin was the highest levels on the dark conditions and significantly higher than light condition.Conclusion： Auxin is not suitable for the active compound production of S.baicalensis.The combination of 0.2 mg·L-1 NAA＋1.0 mg·L-1 KT promotes the accumulation of baicalin.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期127-130,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国博士后基金(20090450559)
国家中医药管理局项目(06-07ZP51)
国家工程实验室项目(2010ZHSH001)
关键词
外源生长素
黄芩
悬浮细胞
有效成分
内源激素
Auxin
Scutellaria baicalensis
suspension cell
active compound
endogenous hormones
