摘要
目的:比较钠石灰和低压氧仪2种不同缺氧方式诱导的慢性间歇性缺氧小鼠模型的整体行为变化,为进一步构建气阴两虚证动物模型奠定基础。方法:分别采用氧分压从21%逐渐降至6%,平均下降速度为0.5%/min～1%/min的钠石灰缺氧法和氧分压在短时间内骤降后维持在7%～8%的低压氧仪缺氧法,考察2种缺氧模式对小鼠体重,摄食量,自发活动以及心率、T波等气阴两虚临床相关指标变化的影响。结果:钠石灰和低压氧仪2种不同的缺氧方式均能够导致小鼠体重下降;摄食量减少;行走格数减少,直立次数增加等自发活动改变;心率加快,T波持续抬高等心电图变化,二者对各项指标的影响呈基本一致的趋势,其结果具有显著相关性。结论:钠石灰和低压氧仪2种缺氧方式诱导的慢性间歇性缺氧小鼠模型均能够模拟形体消瘦,食欲不振,体倦乏力,烦躁,心悸,脉细数等气阴两虚证的主要临床特征。
Objective：To establish foundation for constructing a suitable animal model of deficiency of both Qi and Yin syndrome（DQYS） induced by two types of chronic intermittent hypoxia（soda lime and low-pressure oxygen controller） by comparison the whole behavior in mice.Method： The two modes of oxygen scarcity,including oxygen pressure declining continuously from 21% to 7%-8% with the average speed of 0.5%/min-1% per minute in airtight wide-mouthed bottle and oxygen pressure falling abruptly within short period and then keeping in the level of 7%-8%,were adopted respectively to observe the time-course changes in body weight,food intake,locomotive activity and electrocardiogram respectively.The experiments were designed for mimicing clinical features of DQYS.Result： The mice exposed to two different modes of chronic intermittent hypoxia for certain duration showed body weight loss,food intake decline,locomotive activity change of ambulation decrease and rearing increase,heart rate increase and T wave elevation.There was significant correlation between the results of the two groups.Conclusion： Both types of chronic intermittent hypoxia can simulate the major clinical features of DQYS mainly characterized by tiredness,emaciation,anorexia,fidget,palpitation and rapid pulse,etc.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期171-176,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"十一五"国家科技支撑计划(2008BAI51B03)
中央高校科研专项基金(JKY2009006)
关键词
慢性间歇性缺氧
气阴两虚证
动物模型
低压氧仪
钠石灰
chronic intermittent hypoxia
deficiency of both Qi and Yin syndrome
animal model
low-pressure oxygen controller
soda lime
