摘要
为了解三叶草属抗旱的生理机理，寻找适宜干旱地区种植的三叶草品种，比较了地三叶草（Trifoliumsubterraneum L．）、大花三叶草（rmichelianum Savi）、自：二三叶草（rrepens L．）、高加索三叶草（T.ambiguum M．Bieb．）和草莓三叶草（zfragiferum L．）在人工干旱条件下4wk的生长和生理反应．结果表明，干旱处理下大仡乏叶草、草莓三叶草和白三叶草地上部分干重严重减少，分别降低了70％、66％和64％，而高加索三叶草和地三叶草减少不到50％；高加索三叶草叶片水势降低到-2．0MPa以下，而其他4种三叶草只降低到-1．5MPa左右；高加索三叶草和争莓三叶草叶片光合速率降低25％，而地三叶草、白三叶草和大花三叶草下降35％～63％．此外，干旱条件下，5种供试三叶草叶片中游离脯氨酸含量普遍增加，其中高加索三叶草和白三叶草分别增加到2．5倍和3．1倍．研究显示高加索三叶草在多项重要生长和生理指标中表现出很强的抗旱性，因此认为其可作为干旱地区栽培自叶草的补充或替代种．
In order to reveal physiological mechanisms of clover under drought stress, and to identify clover species suitable for arid areas, five species, namely Trifolium. subterraneum L., T. michelianum Savi., T. repens L., T. ambiguum M. Bieb. and T. fragiferum L. were exposed to simulated drought for 4 weeks. The species＇ growth and physiological responses to drought were studied and compared. The results indicated that the aerial dry matter production of T. michelianum, T..fi＇agiferum and T. repens decreased by 70%, 66% and 64%, respectively, whereas those of T. ambiguum M. Bieb. and T. subterraneum L. decreased by less than 50%. The drought treatment reduced leaf water potential from approximately -1.10 MPa to 2.0 MPa in T. ambiguum. In contrast, the other four species＇ leaf water potential reached approximately -1.5 Mpa under drought. Meanwhile, the leaf photosynthetic rate of T. ambiguum and T. fragiferum declined by 25%, while in the other three clover species it decreased by 35%-63% under drought. In addition, free proline levels in all five clover species increased in response to water deficit, increasing 2.5-fold and 3.1-fold in leaves of T. ambiguum and T. repens, respectively, in comparison to the well-watered treatment. It is concluded that T. ambiguum M. Bieb. displayed drought resistance in a number of key growth and physiological parameters, and could be used as a complement or alternative to T. repens L. in drought-prone pasture environments. Fig clover; 1, Tab 3, Ref22
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第4期580-584,共5页
Chinese Journal of Applied and Environmental Biology
基金
Supported by the Western Foundation of China Scholarship Council(No.063022)
the Youth Foundation of Education Department of Hunan,China(No.03B011)
关键词
三叶草
干旱胁迫
游离脯氨酸
光合作用
水势
Trifolium
drought stress
free proline
photosynthesis
water potential
