摘要
目的:建立测定人血浆中齐拉西酮浓度的方法。方法:采用反相高相液相色谱法,色谱柱为美国Diamonsil C18,流动相为乙腈∶甲醇∶缓冲液(三乙胺5mL加入水1000mL中,冰醋酸调节pH值至3.2)=28∶12∶60,流速为1.3mL·min-1,检测波长为254nm,进样量为20μL。结果:齐拉西酮血药浓度在0.022～2.816μg·mL-1范围内线性关系良好(r=0.9992,n=8),定量下限为0.022μg·mL-1。结论:本方法快速、简便、准确、灵敏度高、重现性好,可以用于齐拉西酮临床研究和血药浓度监测。
OBJECTIVE： To establish the method for the determination of ziprasidone in human plasma. METHODS： RP-HPLC method was adopted. The determination was performed on Diamonsil C18 （250 mmx4.6 mm, 5 μm） with mobile phase consisted of acetonitrile ： methanol ：buffer （triethylamine 5 mL added into water 1 000 mL, pH value adjusted.to 3.2 using glacial acetic acid）（28： 12：60） at the flow rate of 1.3 mL.min^-1.The detection wavelength was set at 254 nm and injection volume was 20 ltL. RESULTS： The linear range of ziprasidone were 0.022-2.816 pg. mL^-1 （r=0.999 2, n=8）. The lowest limit of quantitation was 0.022 pg-mL^-1. CONCLUSIONS： It appears to be a rapid, convenient, accurate, sensitive and reproducible method for clinical research and plasma concentration monitoring of ziprasidone.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第34期3196-3198,共3页
China Pharmacy
基金
2008年河南省高校科技创新团队支持计划项目(2008IRT-STHN008)
