摘要
目的探讨槲皮素对铅致大鼠神经损伤的缓解作用。方法雄性SD大鼠36只,随机分为对照组、醋酸铅组和醋酸铅+槲皮素组。醋酸铅组大鼠采用550 mg/L醋酸铅饮水染毒,醋酸铅+槲皮素干预组大鼠在醋酸铅饮水染毒的同时进行30 mg/kg槲皮素腹腔注射,共8周。应用甩尾实验、热板仪实验和后肢撑力实验测试大鼠的感觉和运动功能;应用morris水迷宫测试学习记忆功能。应用电感耦合等离子体质谱测定血清、海马和皮质中铅水平;同时检测海马和血清中一氧化氮(NO)水平和一氧化氮合酶(NOS)活力。结果与对照组比较,醋酸铅组海马和皮质中铅水平分别增加了29.8和32.3倍,血清中的铅水平增加了1.75倍。醋酸铅组大鼠甩尾时间延长,后肢两掌间距离由对照组的(8.29±1.31)cm增加到(10.67±1.76)cm(P<0.05);morris水迷宫测试结果显示染铅大鼠在目标象停留的时间增加;染铅大鼠海马组织中NO水平下降了24%,神经型NOS(nNOS)的活力也呈下降的趋势(P<0.05);血清NO水平(157.42±39.96)μmol/L高于对照组(99.07±9.80)μmol/L,诱导型NOS(iNOS)活力(23.15±5.26)U/L高于对照组(12.76±4.36)U/L。应用槲皮素干预后,大鼠海马铅水平(6.02±1.21)μg/g显著低于醋酸铅组(8.01±2.02)μg/g;同时大鼠血清中铅水平也明显下降。与醋酸铅组比较,槲皮素干预组大鼠的甩尾时间缩短和两后肢两掌间距离缩短,差异有统计学意义(P<0.05)。海马中NO水平从(0.237±0.061)mmol/g提高到(0.295±0.018)mmol/g,nNOS活力也显著增加(P<0.05)。但未见干预组大鼠血清NO水平和iNOS活力下降。结论槲皮素对铅引起的运动功能损伤有一定缓解作用。
Objective To investigate the alleviative effect of quercetin on nerve damage induced by lead exposure.Methods 36 male SD rats were randomly divided into the control group,lead group and lead＋quercetin group.Rats in lead group were exposed to 550 mg/L lead acetate by drinking water.Rats in lead＋quercetin group were administrated both with quercetin（i.p.）at a dose of 30 mg/kg for 8 weeks and lead drinking water.The sensory and motor nerve function tests,including tail flick test,hot plate test and hindlimb splay test were performed.Morris maze was applied to test learning and memory abilities.Lead concentrations in serum,hippocampus and cortex were detected by ICP-MS.Meanwhile the levels of NO and NOS in hippocampus and serum were measured.Results Compared with those of the control,the lead levels in hippocampus,cortex and serum in lead exposed rats increased by 29.8,32.3 and 1.75 folds respectively.Sensory and motor nerve function test results showed that the periods of tail flick prolonged and the space of hindlimb splay in lead exposed rats increased from（8.29±1.31） cm（control group）to（10.67±1.76） cm（P0.05）.Morris maze test showed that the periods staying in the target zone prolonged in lead exposed rats.NO concentration in hippocampus in lead exposed rats decreased by 24%,while nNOS activity showed a downward trend（P0.05）.Serum NO level in lead exposed rats increased from（99.07±9.80） μmol/L（the control group）to（157.42±39.96） μmol/L and iNOS activity also increased.After quercetin intervention,lead level in hippocampus decreased from（8.01±2.02） μg/g to（6.02±1.21） μg/g and the serum lead level decreased.Compared with those in lead group,the readings of rats tail flick and space of hindlimb splay in quercetin group significantly decreased（P0.05）.While NO and nNOS levels in hippocampus of quercetion administrated rats increased,NO or iNOS activities in serum remained unchanged.Conclusion Quercetin might play a role in alleviating nerve damage induced by lead to some extent.
出处
《中国职业医学》
CAS
北大核心
2011年第4期302-305,共4页
China Occupational Medicine
基金
河北省留学归国人员基金项目(20100320)
关键词
槲皮素
铅
神经行为
海马
大鼠
Quercetin
Lead
Neurobehavior
Hippocampus
Rat
