摘要
目的:研究四川洪雅地区黄连(味连)根茎生物量和有效成分积累的动态变化规律,为确定最佳采收期,实现洪雅地区黄连的GAP种植和质量控制提供理论依据。方法:定期定点采集三至五年生黄连样品,分析黄连根茎生物量变化,采用高效液相色谱法(HPLC)测定根茎药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀和小檗碱的含量。结果:随着黄连生长年限的增长,根茎生物量在不断增加,以四年生黄连根茎生物量增长较快,在10月份达到最高。黄连根茎中有效成分的积累有一定规律。四年生黄连的6种生物碱和总碱要高于三年和五年生黄连。不同月份间,四年生黄连根茎中非洲防己碱、表小檗碱、黄连碱、巴马汀、小檗碱和总碱的含量无显著差异。结论:根据黄连根茎生物量变化及有效成分的积累动态规律,洪雅地区黄连的最佳采收期为10月份的四年生黄连根茎。
Objective： To study the dynamic accumulation of the effective components and biomass of Coptis chinensis,so to provide the experimental date of optimal harvest time for C.chinensis in Hongya county.Method： The samples of three to five years were gathered from the same field and time.The biomass was analyzed by weighed.The jatrorrhizine,columbamine,epiberberine,coptisine,palmatine and berberine in C.chinensis were analyzed by HPLC.Result： With the increasing of years of growth,the rootstalk biomass of C.chinensis was increasing continuously.The biomass growth of four-year-old C.chinensis was the fastest in the year.From September to October was the fastest season of the growth of rootstalk.The dynamic accumulation in rootstalk C.chinensis had regularity in the certain extend.The contents of six alkaloids and all alkaloids in 4-year-old C.chinensis were more than that in 3-years-old and 5-year-old.The contents of six alkaloids were mostly highest in August.From July to December,there is no significant difference in the contents of columbamine,epiberberine,coptisine,palmatine,berberine and all alkaloids in 4-years-old C.chinensis.Conclusion： According to the biomass and the accumulation pattern of the effective components in the C.chinensis,the optimal harvest time is from September to October of 4-year-old C.chinensis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第16期2162-2165,共4页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划重点项目(2007BAI40B00)
国家科技重大专项(2009ZX09308-002)
关键词
洪雅
黄连
生物量
生物碱
Coptis chinensi
biomass
alkaloids
