摘要
目的建立广升麻药材的质量标准。方法分别采用显微观察、薄层色谱法和反相高效液相色谱法,开展广升麻粉末特征鉴别、蜕皮甾酮薄层色谱鉴别及其含量测定的研究。以RP-HPLC法测定广升麻蜕皮甾酮含量,色谱柱为Sepax Sapphire-C18柱(250 mm×4.60 mm,5μm),流动相∶甲醇-水(45∶55),检测波长:248 nm,流速:1.0 mL/min;柱温:30℃。结果广升麻药材粉末菊糖居多,特征性明显;蜕皮甾酮薄层色谱斑点清晰,鉴别特征明显,专属性强;蜕皮甾酮在0.1904μg～2.0940μg间具有良好的线性关系(r=0.9999);平均回收率为99.73%,RSD为2.57%(n=6),重复性RSD为0.64%(n=6)。结论 RP-HPLC测定蜕皮甾酮含量,测定方法操作简便,重复性及稳定性好,结果准确,能有效控制广升麻中蜕皮甾酮的含量,建议推荐为广升麻中蜕皮甾酮的质量控制方法。
Objective To establish the quality standard of Radix Serratulae Chinensis.Methods The micromethod and TLC were used for qualitative identification.Meanwhile,a RP-HPLC analysis was applied for quantitative determination of ecdysterone.The analysis was carried on Sepax Sapphire-C18 column（250 mm×4.60 mm,5 μm）,with mobile phase consisted of methanol-water solution（45：55） at a flow rate of 1.0 mL/min and the detective wavelength was 248 nm,the column temperature is 30℃.Results Kinds of inulins obviously distributed in Radix Serratulae Chinensis,the characteristic of identification by TLC was distinct and highly specific.The linear range of ecdysterone was 0.1904-2.0940 μg（r=0.9999）,the average recovery was 99.73%,RSD was 2.57%（n=6）,and repetition RSD was 0.64%（n=6）.Conclusion RP-HPLC analysis can be applied for determination of ecdysterone,and the method is simple,accurate and reproducible,and suitable for the quality control of Radix Serratulae Chinensis.
出处
《福建中医药大学学报》
2011年第4期38-41,共4页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省中西医结合老年性疾病重点实验室开放课题(2008J1004-42)
陈可冀中西医结合发展基金(CKJ2008079)
福建中医药大学校管课题(X2008010)
