摘要
[目的]快速、有效、大批量地提取藻红蛋白。[方法]以坛紫菜为原料,采用溶胀法、化学试剂法和搅切法3种粗提技术,以藻红蛋白的提取得率、纯度和提取时间为参数对3种方法进行比较。[结果]溶胀法采用0.01mol/LTris-HCl(pH8.0)浸提9h,藻红蛋白得率为9.71mg/g,纯度(A565/A280)为0.45;化学试剂法在0.100mol/LSDS溶液作用4h时,藻红蛋白得率为24.35mg/g,纯度(A565/A280)为0.22左右;搅切法采用搅切速度18000r/min作用6min,藻红蛋白得率达到23.11mg/g,纯度(A565/A280)为0.30。[结论]综合比较,搅切法提取藻红蛋白效率高,藻红蛋白得率和纯度较好,处理量较大,是一种十分有效的粗提方法。
[Objective]The method of rapid,effective and a large amount of phycoerythrin extracted from Porphyra haitanensis was experimented.[Method]The indicator of the yield and purity of phycoerythrin from the Porphyra haitanensis and the extracting time in three kinds of the extraction techniques：swelling,chemical permeabilization and cutting while stirring were compared.[Result]The result showed that the yield of phycoerythrin from the Porphyra haitanensis was 9.71 mg/g,which purity was 0.45,with swelling technique under the condition of the concentration of Tris-HCl：0.01 mol/L,the pH value：8.0 and the extracting time：9 hours;the yield of phycoerythrin was 24.35 mg/g,which purity was about 0.22,with chemical permeabilization under the condition of the concentration of SDS：0.100 mol/L and the action time：4 hours and the yield of phycoerythrin was 23.11 mg/g,which purity was 0.30,with the cutting while stirring method under the condition of the stirring rate：18 000 r/min and the working time：6 minutes.[Conclusion]Based on the integrated comparison,the the cutting while stirring method was best in the extracting efficiency of phycoerythrin including its yield and purity and also a large amount of raw material could be treated at same time,which was an effective method.
出处
《安徽农业科学》
CAS
北大核心
2011年第21期12649-12651,共3页
Journal of Anhui Agricultural Sciences
关键词
坛紫菜
藻红蛋白
破壁技术
Porphyra haitanensis
Phycoerythrin
Extraction
