摘要
目的建立测定降压平片中葛根素的高效液相色谱法。方法采用色谱柱：Diamonsil-C18（4.6 mm×150 mm,5μm）;流动相：甲醇-乙腈-水（6∶8∶86）;检测波长：250 nm,对方中葛根的有效成分葛根素进行含量测定。结果葛根素回归方程为Y=46 512.53X-36 398.96,r=0.9999,在24~96μg/ml浓度范围呈良好的线性关系。平均加样回收率为98.64%,RSD为0.41%（n=6）。结论该法简便可行、重复性好,可以用来控制降压平片的质量。
Objective To establish a method for determination of puerarin in Jiangyaping tablets by high performance liquid chromatography.Methods A Diamonsil-C18 column（4.6 mm×150 mm,5 μm） and mobile phase of methanol-acetonitrile-water（6∶8∶86） were used.The detection wavelength was 250 nm.Results The calibration curve was in good linear relationship over the range of 24-96 μg/ml（r=0.9999）.The average recovery was 98.64%,and RSD was 0.41%（n=4）.Conclusion This method is simple,quick,sensitive,accurate,reproducible and can be used to control the quality of Jiangyaping tablets.
出处
《解放军药学学报》
CAS
2011年第4期348-349,共2页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
降压平片
葛根素
高效液相色谱法
含量测定
Jiangyaping tablets
puerarin
high performance liquid chromatography
determination
