摘要
目的:建立HPLC-MS法测定人血浆中埃索美拉唑含量,为药动学研究提供帮助。方法:血浆蛋白经乙腈溶液处理后,采用Zorbax SB-C18色谱柱(2.1 mm×50 mm,5μm),柱温30℃,以乙腈-0.1%甲酸为流动相,流速0.3 ml.min-1,通过电喷雾离子源,以多反应监测方式(MRM)进行离子检测,通道为m/z346.0→197.7(埃索美拉唑)结果:血浆中埃索美拉唑在5 ng.ml-1～1000 ng.ml-1浓度范围内线性关系良好,最低检测限为5 ng.ml-1。结论:该方法操作简便,分析快速,适用于人血浆中埃索美拉唑的检测。
Objective：To develop a HPLC-MS method for the determination of esomeprazole in human plasma,and help the pharmacokinetics investigation of esomeprazole.Methods：The plasma sample were simply pretreated by protein precipitation with acetonitrile,and the concentration of esomeprazole was determined by HPLC-MS using Zorbax SB-C18 column（2.1 mm×50 mm,5 μm） as separation column with column temperature of 30℃.The mobile phase consisted of acetonitrile-0.1% formic acid solution at a rate of 0.3 ml·min-1,with electrospray positive ionization（ESI＋） and multiple reaction monitoring（m/z 346.0→197.7for esomeprazole）.Results：The calibration curve was linear within the range of 5 ng·ml-1～1000 ng·ml-1.The limit of detection was 5 ng·ml-1.Conclution：The method is convenient,rapid and sensitive.It can be used for the determination of esomeprazole in human plasma.
出处
《中国卫生检验杂志》
CAS
2011年第8期1875-1877,共3页
Chinese Journal of Health Laboratory Technology
