摘要
目的探讨采用血管内穿刺法制作兔脑血管痉挛(CVS)模型的方法。方法对78只新西兰大白兔采用血管内穿刺法产生蛛网膜下腔出血(SAH),制作CVS模型。存活兔随机分成3、12h,1、2、3、7和14 d组,共7组,设置正常组及对照组(无蛛网膜下腔出血)各5只。每只兔术前、术后常规行头颅CT检查,处死后常规行HE染色观察后交通动脉及基底动脉管腔内直径及管壁厚度改变。结果 78只兔中,成功制成模型并获取标本45只(SAH组35只,对照组10只),模型制作成功率为57.7%。SAH组后交通动脉及基底动脉管腔内直径在SAH后12 h分别缩小了45.6%和52.3%。随后血管直径呈双相期改变,管腔直径在第7天再次明显缩小,分别下降了31.2%和48.6%。结论采用经股动脉行血管内穿刺法制作的兔CVS模型病理改变明显,是一种适合研究CVS发生机制的动物模型,虽然动物死亡率高,但是注意操作的细节和应用新型合适材料,能提高模型制作的成功率。
Objective To investigate the method of establishing cerebral vasospasm （CVS） models in rabbits by using endovascular puncture technique. Methods Endovascular puncture procedure was performed in 78 New Zealand white rabbits to produce subarachnoid hemorrhage （SAH）. The survival rabbits were randomly divided into seven groups （3 h, 12 h, 1 d, 2 d, 3 d, 7 d and 14 d）, with five rabbits in each group for both study group （SAH group） and control group. Cerebral CT scanning was carried out in all rabbits both before and after the operation. The inner diameter and the thickness of vascular wall of both posterior communicating artery （PcoA） and basilar artery （BA） were determined after the animals were sacrificed, and the results were analyzed. Results Of 78 experimental rabbits, CVS model was successfully established in 45, including 35 of SAH group and 10 of control subgroup. The technical success rate was 57.7%. Twelve hours after the procedure, the inner diameter of PcoA and BA in SAH group was decreased by 45.6%and 52.3%, respectively, when compared with these in control group. The vascular narrowing showed biphasic changes, the inner diameter markedly decreased again at the 7th day when the decrease reached its peak to 31.2% and 48.6%, respectively. Conclusion Endovascular puncture technique is an effective method to establish CVS models in rabbits. The death rate of experimental animals can de decreased if new interventional material is used and the manipulation is carefully performed.
出处
《介入放射学杂志》
CSCD
北大核心
2011年第8期637-640,共4页
Journal of Interventional Radiology
关键词
脑血管痉挛
动物模型
血管内穿刺
cerebral vasospasm
animal model
endovascular puncture
