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依曲替酸对HaCaT细胞维生素D受体表达的影响

Effects of Acitretin on Expression of VDR in HaCaT Cells
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摘要 目的探索依曲替酸治疗银屑病的机制。方法免疫组化法检测银屑病皮损和正常表皮维生素D受体(VDR)蛋白表达;Western印迹法半定量检测依曲替酸对HaCaT细胞VDR蛋白表达的影响。结果银屑病皮损VDR蛋白表达水平(33.41±2.22)明显高于正常皮肤(14.87±3.18),差异有统计学意义(P<0.01);不同浓度依曲替酸作用HaCaT细胞24h,48h和72h后细胞VDR蛋白表达均较阴性对照组降低,呈明显的时间和剂量依赖性。结论银屑病皮损VDR蛋白表达异常,依曲替酸可降低HaCaT细胞VDR蛋白表达。 Objective To explore the antipsoriatic mechanism of acitretin. Methods The expression of VDR protein were observed both in psoriatic epidermis and normal skin by immunohistochemical method. VDR protein was half quantitatively analyzed by western blot assay after culturing HaCaT cells exposed to aeitretin. Re- sults lmmunohistochemical results showed that expression level of VDR protein in psoriatic lesion（ 33.41 ± 2.22） was higher than that in normal epidermis （14.87 ±3. 18 ） （P 〈 0.01 ）. Level of VDR protein in HaCaT cells was decreased after treated by acitretin with a time and dose. Conclutions The expression of VDR protein in psoriatic lesion is abnormal. Acitretin can decrease expression of VDR protein in HaCaT cells.
作者 侯素春 刘晓明 齐清会 张振颖
机构地区 深圳市第二中医院皮肤科 大连医科大学附属一院皮肤科 大连医科大学附属一院外科
出处 《中国皮肤性病学杂志》 CAS 北大核心 2011年第9期671-674,共4页 The Chinese Journal of Dermatovenereology
关键词 银屑病 维生素D受体 HACAT细胞 依曲替酸 Psoriasis VDR HaCaT cell Acitretin
分类号 R758.63 [医药卫生—皮肤病学与性病学]
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中国皮肤性病学杂志
2011年 第9期

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