摘要
背景:纳米材料构建具有生物活性的组织工程骨,可以很好的模仿体内细胞外基质的结构,有利于细胞黏附、生长。目的:评价新型仿生壳聚糖/胶原纳米纤维支架与SD大鼠骨髓基质干细胞的体外相容性。方法:分离培养SD大鼠骨髓基质干细胞,流式细胞分析法对细胞表面抗原进行检测;相差显微镜观察细胞形态。聚电解质共凝聚技术制作仿生壳聚糖/胶原纳米纤维支架,取生长良好的P3代,与仿生壳聚糖/胶原纳米纤维支架体外联合诱导培养,通过细胞贴壁率、生长曲线、细胞活力、周期、细胞Ⅰ型胶原染色、扫描电镜观察综合评价材料与细胞的相容性。结果与结论:骨髓基质干细胞可在体外分离扩增,表达CD29、CD44和CD106,不表达CD34和CD45,细胞形态为长梭形,仿生壳聚糖/胶原纳米纤维平均孔径为150μm,与骨髓基质干细胞有较好的黏附性。提示骨髓基质干细胞可在体外长期、稳定培养;是理想的组织工程种子细胞;仿生壳聚糖/胶原纳米纤维与骨髓基质干细胞有良好的相容性,可用来做组织工程生物材料。
BACKGROUND： Nano-materials to build tissue engineered bone with bioactivity can simulate the structure of the extracellular matrix that is conducive to cell adhesion and growth. OBJECTIVE： To investigate the in vitro histocompatibility of nano chitosan-sodium／collagen （nano-CS／COL） scaffolds. METHODS： Bone marrow mesenchymal stem cells （BMSCs） were isolated from SD rats and cultured, and then were identified by morphology observations with the optical microscope and the exterior antigen through the flow cytometry method. Nano-CS／COL biomimetic scaffolds were prepared through the polyelectrolyte complex coacervation, then were co-cultured with BMSCs. The cellular compatibility was detected through the adherent rate, growth curve, cell livingness and cell cycle, dyeingⅠcollagen and morphology observations by the scanning electron microscope. RESULTS AND CONCLUSION： The BMSCs could be obtained and cultured, express CD29, CD44, CD106, not CD34, CD45. The diameter of the nano-CS-COL scaffold was 150 μm with good histocompatibility to BMSCs. The BMSCs can be obtained and cultured stably for a long term in vitro, which are the ideal choice for tissue engineering. Nano-CS/COL scaffolds can be used as tissue engineering biomaterials for their good histocompatibility.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第29期5384-5388,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
