摘要
目的:大桑菊合剂为医院制剂,由桑叶、菊花、薄荷、连翘、鱼腥草等中药经乙醇提取制成,为完善大桑菊合剂的制备工艺,对该制剂的制备工艺进行初步的研究。方法:①实验设计:以加水量、提取时间、提取次数和醇沉浓度为考察因素,各因素均取3水平,采用正交试验L(934)优选制备工艺。②含量测定方法:以大桑菊饮合剂中总黄酮的含量为指标,以芦丁为标准品,利用紫外分光光度计于503nm下测定吸光值,计算样品中总黄酮的含量,以此作为筛选大桑菊合剂最佳制备工艺的重要指标。结果:最佳制备工艺为不浸泡,加8倍水,提取3次,每次1小时。结论:经正交设计优化的大桑菊合剂的中药制备工艺合理、可行。
Objective： DaSangJu mixture is preparation for the hospital, it is consisted by Folium moil, chrysanthemi flos, Herba menthae, Fructus forsythiae, Bulbus fritillariae thunbergii and other traditional Chinese medicine, and it is extraction by the water, the main object of this study is in order to optimize the extraction of Dasangju mixture. Methods： Experimental Design： The adding amount of water, extracted times, the extraction numbers and ethanol precipitation the concentration of study factors, all factors are taken 3 levels, the use oforthogonal L9 （34） preferred preparation process.Determination： The DaSangJu mixture by the index of total flavonoids, rutin as the standard, by UV spectrophotometer at 503nm absorbance was measured to calculate the total flavonoid content in the sample, as a screening DaSangJu mixture An important indicator of the best preparation. Results： Best Preparation： Do not soak, plus 8 folds of water, extracted 3 times, each for 1 hours. Conclusions： The orthogonal design of DaSangJu mixture of traditional Chinese medicine preparation process is reasonable and feasible.
出处
《现代生物医学进展》
CAS
2011年第17期3359-3361,共3页
Progress in Modern Biomedicine
基金
广东省2009年建设中医药强省科研课题(2009140)
关键词
正交试验
制备工艺
大桑菊合剂
Orthogonal design
Preparation
DaSangJu mixture
