摘要
探讨高值血清CA19-9稀释后测定的结果与临床影响因素。对高值血清CA19-9用不同的稀释液(去离子水,Dil稀释液,健康人混合低值血清),不同稀释倍数(1∶2;1∶5;1∶10;1∶20)经手工稀释测定和仪器自动稀释检测。结果表明:用Dil稀释液进行稀释,手工法和仪器自动稀释法呈明显正相关;3种稀释液稀释高值血清CA19-9均无显著性差异(P>0.05);各稀释物在不同的稀释倍数之间结果无显著性差异(P>0.05)。其中试剂盒检测线性范围内高值血清CA19-9稀释测定结果与直接测定值有较好的相关性。用3种稀释液可以满足临床稀释要求,而仪器自动稀释法更有助于操作的自动化,减少人为操作的随机误差。
To explore the diluted results and clinical influencing factor of high CA19-9 in human serum.The human high serum CA19-9 were measured by deionized water,Dil,and low serum,and by different dilution ratio(1∶2,1∶5,1∶10,1∶20) in manual and instrumental automatic dilution.There was a positive correlation between manual and instrumental dilution with Dil.There was no significant difference in three different diluents and dilution ratio in high serum CA19-9.The three diluents were suitable for clinical analysis,and Dil was more favorable for automation in laboratory and for preventing from accidental error.
出处
《标记免疫分析与临床》
CAS
2011年第4期265-267,共3页
Labeled Immunoassays and Clinical Medicine
基金
国家科技支撑计划(2009BAI86B05)