摘要
为了更好的利用乌克兰小麦品种资源,并了解引进品种的品质,采用SDS-PAGE和A-PAGE技术,对从乌克兰引进小麦材料的高分子量麦谷蛋白亚基(HMW-GS)和醇溶蛋白亚基的组成进行分析。结果表明,在16个普通小麦品种中,由Glu-1位点控制的高分子量亚基组合类型共有7种,最常见的是(1,7+8,5+10)占37.5%,其次是(1,2+12,6+8)和(1,7+9,5+10),各占18.75%,其中Glu-A1位点有3种等位变异,以1亚基为主(75%);Glu-B1位点有3种等位变异,以7+8为主(43.75%);Glu-D1位点有3种等位变异,以5+10为主(68.75%)。醇溶蛋白方面,从供试材料的6个位点中,共鉴定了33个不同的醇溶蛋白等位基因,41条迁移率不同的醇溶蛋白带纹,其中Gli-A1,Gli-B1和Gli-D1分别有6,5,5个等位基因;Gli-A2,Gli-B2和Gli-D2各有6,5,6个等位基因,优质亚基Gli-B1b出现频率较高(43.75%),这些材料有可能会成为比较有价值的品质改良中间材料。
In order to make better use of Ukrainian wheat varieties,understand the quality of introduced species,the HMW-GS and gliadin subunits compositions of 16 wheat cultivars from Ukraine were analyzed by SDS-PAGE and A-PAGE.The results showed that a total of seven subunit combinations were examined on Glu-1 loci,and（1,7＋8,5＋10） were the major combination type with frequencies of 37.5%,followed by combinations（1,2＋12,6＋8） and（1,7＋9,5＋10）,the frequencies were 18.75%.Three kinds of HMW-GS on Glu-A1 were examined,the frequency of 1 was the highest（75%）.There were three kinds of HMW-GS on Glu-B1,and subunits 7＋8 were the major types with frequencies of 43.75%.Three types were detected on Glu-D1,and 5＋10 appears more frequently（68.75%）.Aspects of gliadin,33 gliadin band patterns were encoded by Gli-1 locus and there were 41 protein bands with different mobility.6,5,5 alleles were at Gli-A1,Gli-B1,Gli-D1,and there were 6,5,6 alleles at Gli-A2,Gli-B2,Gli-D2.Good-quality gliadin alleles Gli-B1b was the most frequencies alleles（43.75%）.Maybe these varieties can be used as a valuable material for breeding.
出处
《中国农学通报》
CSCD
北大核心
2011年第21期32-38,共7页
Chinese Agricultural Science Bulletin
基金
国家科技支撑计划"牧草倍性育种技术研究"(2008-BADB3B03)
北京市国际合作项目"几种重要种质资源的引进及品种改良"(2007N16)
